Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX2 antibody [9-9-3]

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Monodelphis domestica Tissue sections (Spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Triton X-100 in PBS for 1 hour
Specification
Spinal cord
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Paraformaldehyde

Other product details

Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1%BSA+1%goat serum+0,1% Tween 20 in PBS
Dilution
1/500

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Goat anti-Mouse IgG1 cross-adsorbed (Thermo Fisher
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
Works on spinal cord from neonatal P5 opossums (Monodelphis domestica)

Jelena Ban

Verified customer

Submitted Mar 25 2019

Sign up