Overview

  • Product name

  • Description

    Rabbit polyclonal to SOX2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Cat, Monkey
  • Immunogen

    Recombinant fragment within Human SOX2 (internal sequence). The exact sequence is proprietary.
    Database link: P48431

  • Positive control

    • WB: Recombinant Human SOX2 protein (ab79950), NT2D1 whole cell lysate. Human and mouse embryonic stem cell lysate. ICC/IF: Human and mouse embryonic stem cells. A549 cells. IHC-P: Human Cal27 xenograft tissue. Flow Cytometry: Human embryonic stem cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab137385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/500 - 1/3000. Predicted molecular weight: 34 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)
ICC/IF 1/50 - 1/500.

Target

  • Function

    Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • Involvement in disease

    Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Post-translational
    modifications

    Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANOP3 antibody
    • cb236 antibody
    • Delta EF2a antibody
    • lcc antibody
    • MCOPS3 antibody
    • MGC148683 antibody
    • MGC2413 antibody
    • RGD1565646 antibody
    • Sex determining region Y box 2 antibody
    • SOX 2 antibody
    • Sox2 antibody
    • SOX2_HUMAN antibody
    • SRY (sex determining region Y) box 2 antibody
    • SRY box containing gene 2 antibody
    • SRY related HMG box 2 antibody
    • SRY related HMG box gene 2 antibody
    • SRY-box 2 antibody
    • Transcription factor SOX 2 antibody
    • Transcription factor SOX-2 antibody
    • ysb antibody
    see all

Images

  • Anti-SOX2 antibody (ab137385) at 1/1000 dilution + Human ESC whole cell lysate
    at 20 µg

    Predicted band size: 34 kDa



    10% SDS-PAGE gel

  • Formalin-fixed, paraffin-embedded Cal27 xenograft tissue stained for SOX2 with ab137385 at a 1/100 dilution in immunohistochemical analysis.

  • Human embryonic stem cells stained for SOX2 (green) using ab137385 (1/160 dilution) in ICC/IF. Lower panel is costained with Hoechst 33342.

  • Flow cytometric analysis of human embryonic stem cells, labelling SOX2 with ab137385 at 1/100 dilution (purple) compared to a rabbit IgG control (black).

  • Anti-SOX2 antibody (ab137385) at 1/1000 dilution + Mouse ESC whole cell lysate at 20 µg

    Predicted band size: 34 kDa



     12% SDS-PAGE gel

  • Anti-SOX2 antibody (ab137385) at 1/3000 dilution + NT2D1 whole cell lysate
    at 30 µg

    Predicted band size: 34 kDa



    10% SDS-PAGE gel

  • Mouse embryonic stem cells stained for SOX2 (green) using ab137385 (1/160 dilution) in ICC/IF. Lower panel is costained with Hoechst 33342.

  • Paraformaldehyde-fixed A549 cells stained for SOX2 (green) using ab137385 (1/200 dilution) in ICC/IF. Lower panel is merged with a DNA probe.

References

This product has been referenced in:

  • Payne SL  et al. Initial cell maturity changes following transplantation in a hyaluronan-based hydrogel and impacts therapeutic success in the stroke-injured rodent brain. Biomaterials 192:309-322 (2019). Read more (PubMed: 30468998) »
  • Xiong Z  et al. LncRNA MALAT1/miR-129 axis promotes glioma tumorigenesis by targeting SOX2. J Cell Mol Med N/A:N/A (2018). Read more (PubMed: 29808528) »
See all 10 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Human cerebral organoid)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12.5% gel)
Loading amount
40 µg
Specification
Human cerebral organoid
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 30 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (neuronal progenitors of human cerebral organoids)
Permeabilization
Yes - Triton X-100
Specification
neuronal progenitors of human cerebral organoids
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 27°C
Fixative
Paraformaldehyde

Radhika Menon

Verified customer

Submitted Jul 05 2017

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