Key features and details
- Goat polyclonal to SOX2
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SOX2 antibody
See all SOX2 primary antibodies
DescriptionGoat polyclonal to SOX2
Tested applicationsSuitable for: Flow Cyt, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Sheep, Chicken
- WB: MCF7 and U-251 MG nuclear cell lysate. ICC/IF: U-251 MG cells. Flow Cyt: MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab239218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 0.1 - 0.3 µg/ml.
1 hour primary incubation is recommended for this product.
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionTranscription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
Involvement in diseaseDefects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
modificationsSumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
- Information by UniProt
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All lanes : Anti-SOX2 antibody (ab239218) at 0.3 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) nuclear cell lysate in RIPA buffer
Lane 2 : U-251 MG (Human brain glioma cell line) nuclear cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Detected by chemiluminescence.
Immunofluorescence analysis of paraformaldehyde fixed U-251 MG (Human brain glioma cell line) cells, permeabilized with 0.15% Triton, labeling SOX2 (green) using ab239218. Primary incubation time 1 hour (10 µg/ml) followed by an Alexa-Fluor®488 secondary antibody (2 µg/ml). Actin filaments were stained with phalloidin (red).
Negative control: Non-immunized goat IgG (10 µg/ml) followed by an Alexa-Fluor®488 secondary antibody (2 µg/ml).
Flow cytometric analysis of paraformaldehyde fixed MCF7 (Human breast adenocarcinoma cell line) cells (blue line), permeabilized with 0.5% Triton, labeling SOX2 (blue line) using ab239218. Primary incubation time 1 hour (10 µg/ml) followed by an Alexa-Fluor®488 secondary antibody (1 µg/ml).
IgG control: Non-immunized goat IgG (black line) followed by an Alexa-Fluor®488 secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab239218 has not yet been referenced specifically in any publications.