Overview

  • Product name

  • Description

    Goat polyclonal to SOX2
  • Host species

    Goat
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Sheep, Chicken
  • Immunogen

    Synthetic peptide corresponding to Human SOX2 aa 2-16 (N terminal) (Cysteine residue). NP_003097.1
    Sequence:

    YNMMETELKPPGPQQ(c)


    Database link: P48431

  • Positive control

    • WB: MCF7 and U-251 MG nuclear cell lysate. ICC/IF: U-251 MG cells. FC: MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab239218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 10 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • Involvement in disease

    Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Post-translational
    modifications

    Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANOP3 antibody
    • cb236 antibody
    • Delta EF2a antibody
    • lcc antibody
    • MCOPS3 antibody
    • MGC148683 antibody
    • MGC2413 antibody
    • RGD1565646 antibody
    • Sex determining region Y box 2 antibody
    • SOX 2 antibody
    • Sox2 antibody
    • SOX2_HUMAN antibody
    • SRY (sex determining region Y) box 2 antibody
    • SRY box containing gene 2 antibody
    • SRY related HMG box 2 antibody
    • SRY related HMG box gene 2 antibody
    • SRY-box 2 antibody
    • Transcription factor SOX 2 antibody
    • Transcription factor SOX-2 antibody
    • ysb antibody
    see all

Images

  • All lanes : Anti-SOX2 antibody (ab239218) at 0.3 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) nuclear cell lysate in RIPA buffer
    Lane 2 : U-251 MG (Human brain glioma cell line) nuclear cell lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.


    Detected by chemiluminescence.

  • Immunofluorescence analysis of paraformaldehyde fixed U-251 MG (Human brain glioma cell line) cells, permeabilized with 0.15% Triton, labeling SOX2 (green) using ab239218. Primary incubation time 1 hour (10 µg/ml) followed by an Alexa-Fluor®488 secondary antibody (2 µg/ml). Actin filaments were stained with phalloidin (red).

    Negative control: Non-immunized goat IgG (10 µg/ml) followed by an Alexa-Fluor®488 secondary antibody (2 µg/ml).

  • Flow cytometric analysis of paraformaldehyde fixed MCF7 (Human breast adenocarcinoma cell line) cells (blue line), permeabilized with 0.5% Triton, labeling SOX2 (blue line) using ab239218. Primary incubation time 1 hour (10 µg/ml) followed by an Alexa-Fluor®488 secondary antibody (1 µg/ml).

    IgG control: Non-immunized goat IgG (black line) followed by an Alexa-Fluor®488 secondary antibody.

References

ab239218 has not yet been referenced specifically in any publications.

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