Overview

  • Product name

  • Description

    Rabbit polyclonal to SOX2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Sheep, Chicken
  • Immunogen

    Synthetic peptide within Human SOX2 aa 250-300. The exact sequence is proprietary. NP_003097.1
    Database link: P48431

  • Positive control

    • WB: H520 and HEK-293T whole cell lysate. IP: SOX2 IP in H520 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • Involvement in disease

    Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Post-translational
    modifications

    Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANOP3 antibody
    • cb236 antibody
    • Delta EF2a antibody
    • lcc antibody
    • MCOPS3 antibody
    • MGC148683 antibody
    • MGC2413 antibody
    • RGD1565646 antibody
    • Sex determining region Y box 2 antibody
    • SOX 2 antibody
    • Sox2 antibody
    • SOX2_HUMAN antibody
    • SRY (sex determining region Y) box 2 antibody
    • SRY box containing gene 2 antibody
    • SRY related HMG box 2 antibody
    • SRY related HMG box gene 2 antibody
    • SRY-box 2 antibody
    • Transcription factor SOX 2 antibody
    • Transcription factor SOX-2 antibody
    • ysb antibody
    see all

Images

  • All lanes : Anti-SOX2 antibody (ab245292) at 0.04 µg/ml

    Lane 1 : H520 whole cell lysate at 50 µg
    Lane 2 : H520 whole cell lysate at 15 µg
    Lane 3 : H520 whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Exposure time: 30 seconds
  • SOX2 was immunoprecipitated from H520 whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245292 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitate using ab245292 at 1 μg/ml.
     
    Lane 1: ab245292 IP in H520 whole cell lysate.
    Lane 2: Control IgG in H520 whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 1 second.

References

ab245292 has not yet been referenced specifically in any publications.

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