Key features and details
- Rabbit polyclonal to SOX2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SOX2 antibody
See all SOX2 primary antibodies
DescriptionRabbit polyclonal to SOX2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Sheep, Chicken
Synthetic peptide within Human SOX2 aa 250-300. The exact sequence is proprietary. NP_003097.1
Database link: P48431
- WB: H520 and HEK-293T whole cell lysate. IP: SOX2 IP in H520 whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245292 was affinity purified using an epitope specific to SOX2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionTranscription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
Involvement in diseaseDefects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
modificationsSumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
- Information by UniProt
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All lanes : Anti-SOX2 antibody (ab245292) at 0.04 µg/ml
Lane 1 : H520 whole cell lysate at 50 µg
Lane 2 : H520 whole cell lysate at 15 µg
Lane 3 : H520 whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 30 seconds
SOX2 was immunoprecipitated from H520 whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245292 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitate using ab245292 at 1 μg/ml.
Lane 1: ab245292 IP in H520 whole cell lysate.
Lane 2: Control IgG in H520 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 1 second.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245292 has not yet been referenced specifically in any publications.