Anti-SOX2 antibody (ab245293)
- Datasheet
- References
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Overview
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Product name
Anti-SOX2 antibody
See all SOX2 primary antibodies -
Description
Rabbit polyclonal to SOX2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Chicken, Xenopus laevis, Xenopus tropicalis
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Immunogen
Synthetic peptide within Human SOX2 aa 295-317. The exact sequence is proprietary. NP_003097.1
Database link: P48431 -
Positive control
- WB: H520 and HEK-293T whole cell lysate. IP: SOX2 IP in H520 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245293 was affinity purified using an epitope specific to SOX2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab245293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. | |
| IP | Use at 2-5 µg/mg of lysate. |
Target
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Function
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. -
Involvement in disease
Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Post-translational
modificationsSumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 396105 Chicken
- Entrez Gene: 6657 Human
- Entrez Gene: 398000 Xenopus laevis
- Omim: 184429 Human
- SwissProt: P48430 Chicken
- SwissProt: P48431 Human
- SwissProt: O42569 Xenopus laevis
- Unigene: 518438 Human
see all -
Alternative names
- ANOP3 antibody
- cb236 antibody
- Delta EF2a antibody
see all
Images
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Western blot - Anti-SOX2 antibody (ab245293)All lanes : Anti-SOX2 antibody (ab245293) at 0.04 µg/ml
Lane 1 : H520 whole cell lysate at 50 µg
Lane 2 : H520 whole cell lysate at 15 µg
Lane 3 : H520 whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 10 seconds -
Immunoprecipitation - Anti-SOX2 antibody (ab245293)SOX2 was immunoprecipitated from H520 whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245293 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitate using ab245293 at 1 μg/ml.
Lane 1: ab245293 IP in H520 whole cell lysate.
Lane 2: Control IgG in H520 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 1 second.
Datasheets and documents
References
ab245293 has not yet been referenced specifically in any publications.
