Anti-SOX2 antibody (ab97959)
Key features and details
- Rabbit polyclonal to SOX2
- Suitable for: ICC, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SOX2 antibody
See all SOX2 primary antibodies -
Description
Rabbit polyclonal to SOX2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Horse, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Quail, Rhesus monkey, Rainbow trout, Spotted catshark, Thornback ray
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Immunogen
Synthetic peptide corresponding to Human SOX2 aa 300 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab97974) -
Positive control
- ICC: NCCIT and NIH/3T3 cells. Dissociated induced pluripotent stem cells from mouse embryonic fibroblasts. Mouse embryonic stem cells. WB: NCCIT, IOUD2, HUES7, F9 and MCF7 whole cell lysate. IHC:Human brain glioma.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1 mg/ml may have BSA added as a stabilizing agent. If you would like information about the formulation of a specific lot, please contact our Scientific Support Team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Applications
Our Abpromise guarantee covers the use of ab97959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use at an assay dependent concentration. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody.
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| IHC-P | Use a concentration of 1 mg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 34,40 kDa (predicted molecular weight: 34 kDa). Recombinant Human SOX2 protein (ab80520) can be used as a positive control in WB. |
Target
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Function
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. -
Involvement in disease
Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Post-translational
modificationsSumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 396105 Chicken
- Entrez Gene: 784383 Cow
- Entrez Gene: 6657 Human
- Entrez Gene: 20674 Mouse
- Entrez Gene: 407739 Pig
- Entrez Gene: 499593 Rat
- Entrez Gene: 398000 Xenopus laevis
- Entrez Gene: 378723 Zebrafish
see all -
Alternative names
- ANOP3 antibody
- cb236 antibody
- Delta EF2a antibody
see all
Images
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Immunohistochemistry (Floating vibratome sections) - Anti-SOX2 antibody (ab97959)
IHC image of SOX2 staining in Human brain glioma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97959, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunocytochemistry - Anti-SOX2 antibody (ab97959)ab97959 staining SOX2 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab97959 at 0.1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Immunocytochemistry - Anti-SOX2 antibody (ab97959)Cell line: NCCIT (human pluripotent embryonal carcinoma)
Target AbID: Ab97959 anti-Sox2, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody was used.
Counterstain AbID: Ab7291 anti-Tubulin (Rabbit mAb), 97959
Fixative: 4% PFA
Permeabilisation: 0.1% Triton-X
Nuclear counter stain: DAPI
Comments: Confocal image showing negative staining on NCCIT cells
Target primary antibody dilution: 1:500
Target secondary antibody dilution: 1:1000 (2ug/mL)
Counterstain primary antibody dilution: 1:1000 (1ug/mL)
Counterstain secondary antibody dilution: 1:1000 (2ug/mL)
Negative control 1 primary antibody dilution: 1:500 (Ab97959)
Negative control 1 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150120)
Negative control 2 primary antibody dilution: 1:1000 (1ug/mL) (Ab7291)
Negative control 2 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150077)
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Western blot - Anti-SOX2 antibody (ab97959)All lanes : Anti-SOX2 antibody (ab97959) at 1 µg
Lane 1 : NCCIT (Human embryonic carcinoma cell line) Whole Cell Lysate
Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : C6 (Rat glial tumor cell line) Whole Cell Lysate
Lane 5 : Hippocampus (Rat) Tissue Lysate
Lane 6 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Blots were developed with goat anti-rabbit IgG (H+L) and goat anti-mouse IgG (H+L) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Green signal from target - ab97959 observed at 40 kDa
Red signal from loading control ab9484 (GAPDH) observed at 37 kDa -
Immunocytochemistry - Anti-SOX2 antibody (ab97959)Cell line: NIH/3T3 (mouse embryonic fibroblast cell line)
Target AbID: Ab97959 anti-Sox2, used Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody
Counterstain AbID: Ab7291 anti-Tubulin (Rabbit mAb), Ab150120 AlexaFluor®594 Goat anti-Mouse secondary
Fixative: 4% PFA
Permeabilisation: 0.1% Triton-X
Nuclear counter stain: DAPI
Comments: Confocal image showing negative staining on NIH/3T3 cells
Target primary antibody dilution: 1:500
Target secondary antibody dilution: 1:1000 (2ug/mL)
Counterstain primary antibody dilution: 1:1000 (1ug/mL)
Counterstain secondary antibody dilution: 1:1000 (2ug/mL)
Negative control 1 primary antibody dilution: 1:500 (Ab97959)
Negative control 1 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150120)
Negative control 2 primary antibody dilution: 1:1000 (1ug/mL) (Ab7291)
Negative control 2 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150077)
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Immunocytochemistry - Anti-SOX2 antibody (ab97959)Muratore CR et al. Comparison and Optimization of hiPSC Forebrain Cortical Differentiation Protocols. PLoS One 9:e105807 (2014). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ICC/IF analysis of dissociated induced pluripotent stem cells from mouse embryonic fibroblasts stained for SOX-2 (Red) using ab97959. TOPRO (purple). Scale bar=100 μm
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Immunocytochemistry - Anti-SOX2 antibody (ab97959)ICC/IF image of ab97959 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97959, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-SOX2 antibody (ab97959)All lanes : Anti-SOX2 antibody (ab97959) at 1 µg/ml
Lane 1 : IOUD2 (Mouse embryonic stem cell) Whole Cell Lysate
Lane 2 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 39 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Protocols
References (389)
ab97959 has been referenced in 389 publications.
- Ballabio C et al. Modeling medulloblastoma in vivo and with human cerebellar organoids. Nat Commun 11:583 (2020). PubMed: 31996670
- Wu H et al. MSC-induced lncRNA HCP5 drove fatty acid oxidation through miR-3619-5p/AMPK/PGC1a/CEBPB axis to promote stemness and chemo-resistance of gastric cancer. Cell Death Dis 11:233 (2020). PubMed: 32300102
- Chen H et al. Regeneration of pulpo-dentinal-like complex by a group of unique multipotent CD24a+ stem cells. Sci Adv 6:eaay1514 (2020). PubMed: 32284993
- Huang L et al. Long non-coding RNA NCK1-AS1 promotes the tumorigenesis of glioma through sponging microRNA-138-2-3p and activating the TRIM24/Wnt/ß-catenin axis. J Exp Clin Cancer Res 39:63 (2020). PubMed: 32293515
- Hwang I et al. CIC is a critical regulator of neuronal differentiation. JCI Insight 5:N/A (2020). PubMed: 32229723
