Anti-SOX2 antibody (ab97959)
Rabbit polyclonal SOX2 antibody. Validated in WB, IP, IHC, ICC/IF and tested in Mouse, Rat, Horse, Chicken, Human, Pig, Xenopus laevis, Zebrafish, Quail, Rainbow trout. Cited in 231 publication(s).
- Datasheet
- References (320)
- Protocols
Overview
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Product name
Anti-SOX2 antibody
See all SOX2 primary antibodies -
Description
Rabbit polyclonal to SOX2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, IP, WB, IHC - Wholemount, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Horse, Chicken, Human, Pig, Xenopus laevis, Zebrafish, Quail, Rainbow trout, Spotted catshark, Thornback ray
Predicted to work with: Sheep, Cow, Rhesus monkey
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Immunogen
Synthetic peptide corresponding to Human SOX2 aa 300 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab97974) -
Positive control
- ICC/IF: NCCIT, NIH/3T3, mouse embryonic stem cells. IHC-P: Mouse E13 spinal cord, zebrafish developing retina, chicken E7 brain, quail developing retina - E9, spotted catshark (Eye, staining of proliferating cell nuclei), rat brain. WB: NCCIT whole cell lysate, F9 whole cell lysate, MCF7 whole cell lysate, C6 whole cell lysate, rat hippocampus tissue lysate, rat spinal cord tissue lysate, IOUD2 whole cell lysate, HUES7 whole cell lysate. IHC -Wholemount: Xenopus laevis embryo, mouse embryo. IP: ES cells
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General notes
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1 mg/ml may have BSA added as a stabilizing agent. If you would like information about the formulation of a specific lot, please contact our Scientific Support Team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Applications
Our Abpromise guarantee covers the use of ab97959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/1000 - 1/2000. | |
| ICC/IF | Use a concentration of 1 µg/ml. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody. |
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| IP | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 34,40 kDa (predicted molecular weight: 34 kDa). Recombinant Human SOX2 protein (ab80520) can be used as a positive control in WB. |
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| IHC - Wholemount | 1/200. | |
| IHC-Fr | Use at an assay dependent concentration. |
Target
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Function
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. -
Involvement in disease
Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Post-translational
modificationsSumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 396105 Chicken
- Entrez Gene: 784383 Cow
- Entrez Gene: 6657 Human
- Entrez Gene: 20674 Mouse
- Entrez Gene: 407739 Pig
- Entrez Gene: 499593 Rat
- Entrez Gene: 398000 Xenopus laevis
- Entrez Gene: 378723 Zebrafish
see all -
Alternative names
- ANOP3 antibody
- cb236 antibody
- Delta EF2a antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959)Cell line: NCCIT (human pluripotent embryonal carcinoma)
Target AbID: Ab97959 anti-Sox2, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody was used.
Counterstain AbID: Ab7291 anti-Tubulin (Rabbit mAb), 97959
Fixative: 4% PFA
Permeabilisation: 0.1% Triton-X
Nuclear counter stain: DAPI
Comments: Confocal image showing negative staining on NCCIT cells
Target primary antibody dilution: 1:500
Target secondary antibody dilution: 1:1000 (2ug/mL)
Counterstain primary antibody dilution: 1:1000 (1ug/mL)
Counterstain secondary antibody dilution: 1:1000 (2ug/mL)
Negative control 1 primary antibody dilution: 1:500 (Ab97959)
Negative control 1 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150120)
Negative control 2 primary antibody dilution: 1:1000 (1ug/mL) (Ab7291)
Negative control 2 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150077)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (ab97959)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomIHC-P image of SOX2 (ab97959) on E13 mouse spinal cord sections. The sections were fixed in formaldehyde and underwent heat mediated antigen retrieval using citric acid (pH 6). The sections were then blocked in 1% BSA solution for 10 mins at 21°C.
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Western blot - Anti-SOX2 antibody (ab97959)All lanes : Anti-SOX2 antibody (ab97959) at 1 µg
Lane 1 : NCCIT (Human embryonic carcinoma cell line) Whole Cell Lysate
Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : C6 (Rat glioma cell line) Whole Cell Lysate
Lane 5 : Hippocampus (Rat) Tissue Lysate
Lane 6 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Blots were developed with goat anti-rabbit IgG (H+L) and goat anti-mouse IgG (H+L) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Green signal from target - ab97959 observed at 40 kDa
Red signal from loading control ab9484 (GAPDH) observed at 37 kDa -
IHC - Wholemount - Anti-SOX2 antibody (ab97959)This image is courtesy of an anonymous AbreviewIHC - Wholemount of Xenopus laevis embryo labelling SOX2 with ab97959. Sample was incubated with primary antibody (1/250) for 12 hours at 4°C. An Alkaline Phosphatase-conjugated mouse anti-rabbit IgG monoclonal (1/1000) was used as the secondary antibody.
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Immunoprecipitation - Anti-SOX2 antibody (ab97959)This image is courtesy of an anonymous Abreview.Incubation time: 16 hours. Temperature: 4°C. Diluent: 50 mM Tris, 150 mM NaCl. Immuno-precipitation step: Covalently coupled to protein G beads. 1 µg of ab97959 was used per 50 µl of protein G beads suspension. Treatment: DMSO or GlcNAcstatin C (Oga inhibitor) for 24h. Total protein in input: 100 µg.
All lanes : Anti-SOX2 antibody (ab97959) at 1/1000 dilution
Lane 1 : ES cells + DMSO (0.01%)
Lane 2 : ES cells + Oga inhibitor (GlcNAcstatin C, 1uM)
Lane 3 : Differentiated ES cells + DMSO (0.01%)
Lane 4 : Differentiated ES cells + Oga inhibitor (GlcNAcstatin C, 1uM)
Lane 5 : ES cells + Oga inhibitor (GlcNAcstatin C, 1uM), Mock IP
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Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959)Cell line: NIH/3T3 (mouse embryonic fibroblast cell line)
Target AbID: Ab97959 anti-Sox2, used Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody
Counterstain AbID: Ab7291 anti-Tubulin (Rabbit mAb), Ab150120 AlexaFluor®594 Goat anti-Mouse secondary
Fixative: 4% PFA
Permeabilisation: 0.1% Triton-X
Nuclear counter stain: DAPI
Comments: Confocal image showing negative staining on NIH/3T3 cells
Target primary antibody dilution: 1:500
Target secondary antibody dilution: 1:1000 (2ug/mL)
Counterstain primary antibody dilution: 1:1000 (1ug/mL)
Counterstain secondary antibody dilution: 1:1000 (2ug/mL)
Negative control 1 primary antibody dilution: 1:500 (Ab97959)
Negative control 1 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150120)
Negative control 2 primary antibody dilution: 1:1000 (1ug/mL) (Ab7291)
Negative control 2 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150077)
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Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959)Muratore CR et al. Comparison and Optimization of hiPSC Forebrain Cortical Differentiation Protocols. PLoS One 9:e105807 (2014). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ICC/IF analysis of dissociated induced pluripotent stem cells from mouse embryonic fibroblasts stained for SOX-2 (Red) using ab97959. TOPRO (purple). Scale bar=100 μm
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Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959)ICC/IF image of ab97959 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97959, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (ab97959)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomIHC-P image of SOX2 (ab97959) on zebrafish developing retina sections. The sections were fixed in formaldehyde and underwent heat mediated antigen retrieval using citric acid (pH 6). The sections were then blocked in 1% BSA solution for 10 mins at 21°C. The upper section used Abcam's EXPOSE HRP-DAB polyvalent kit (ab80436). The lower section was immunostained using stABCpx-DAB method.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (ab97959)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomIHC-P image of SOX2 (ab97959) on E7 chicken brain sections. The sections were fixed in formaldehyde and underwent heat mediated antigen retrieval using citric acid (pH 6). The sections were then blocked in 1% BSA solution for 10 mins at 21°C. The upper section used Abcam's EXPOSE HRP-DAB polyvalent kit (ab80436). The lower section was immunostained using stABCpx-DAB method.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (ab97959)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomIHC-P image of SOX2 (ab97959) on E9 quail developing retina sections. The sections were fixed in formaldehyde and underwent heat mediated antigen retrieval using citric acid (pH 6). The sections were then blocked in 1% BSA solution for 10 mins at 21°C.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (ab97959)This image is courtesy of an anonymous Abreviewab97959 staining SOX2 in spotted catshark (Scyliorhinus canicula) eye tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/500 in TBS + 1% BSA) for 12 hours at 4°C. An HRP-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (ab97959)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomIHC-P image of SOX2 (ab97959) on adult rat brain sections. The sections were fixed in formaldehyde and underwent heat mediated antigen retrieval using citric acid (pH 6). The sections were then blocked in 1% BSA solution for 10 mins at 21°C. Upper image using EXPOSE HRP-DAB polyvalent kit (ab80436) shows strong, clear immunostaining of many nuclei in the subventricular region of the lateral ventricle ( double-red arrowhead) and also of the ependymal cells lining the ventricle ( black arrowheads). Direction of migration indicated by green arrows.
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IHC - Wholemount - Anti-SOX2 antibody (ab97959)This image is courtesy of an anonymous abreview.IHC-Wholemount image of anti-Sox2 antibody (ab97959) staining on a mouse embryo. This embryo was stained with anti-Sox2 antibody (green) for 48 hours at 4°C. Nuclei stained with DAPI (grey). -
Western blot - Anti-SOX2 antibody (ab97959)All lanes : Anti-SOX2 antibody (ab97959) at 1 µg/ml
Lane 1 : IOUD2 (Mouse embryonic stem cell) Whole Cell Lysate
Lane 2 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 39 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Protocols
References
This product has been referenced in:
- Ton ST et al. Subventricular zone neural precursor cell responses after traumatic brain injury and binge alcohol in male rats. J Neurosci Res 97:554-567 (2019). Read more (PubMed: 30614539) »
- Wang X et al. Efficient generation of human primordial germ cell-like cells from pluripotent stem cells in a methylcellulose-based 3D system at large scale. PeerJ 6:e6143 (2019). Read more (PubMed: 30643676) »
