Application
ChIP
Sample
Mouse Cell lysate - nuclear (mESCs)
Negative control
mESC, SOX2 ChiP-qPCR Myog primers
Specification
mESCs
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control
mESC, SOX2 ChiP-qPCR Nanog promoter region primers. enrichment displayed fold input.
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Additional data
Additional Notes
Used this antibody as a positive control, enriched as expected at the Nanog promoter region
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Michael Larcombe
Verified customer
Submitted Jun 11 2021