Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7 cells lysate)
Gel Running Conditions
Reduced Denaturing (4–20% precast polyacrylamide gel)
Loading amount
10 µg
Specification
MCF7 cells lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Rabbit Immu Conjugation: HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 37 kDa Non-specific: 110, 170 kDa
Additional data
Additional Notes
expected at 34 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted May 16 2022