Overview

  • Product name

    Anti-SOX2 antibody [EPR3130]
    See all SOX2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3130] to SOX2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SOX2 aa 250-350. The exact sequence is proprietary.

  • Positive control

    • NCCIT cell lysate; Human fetal brain, Human normal brain and glioma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
IP 1/10.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
    • Involvement in disease

      Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
    • Sequence similarities

      Contains 1 HMG box DNA-binding domain.
    • Post-translational
      modifications

      Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • ANOP3 antibody
      • cb236 antibody
      • Delta EF2a antibody
      • lcc antibody
      • MCOPS3 antibody
      • MGC148683 antibody
      • MGC2413 antibody
      • RGD1565646 antibody
      • Sex determining region Y box 2 antibody
      • SOX 2 antibody
      • Sox2 antibody
      • SOX2_HUMAN antibody
      • SRY (sex determining region Y) box 2 antibody
      • SRY box containing gene 2 antibody
      • SRY related HMG box 2 antibody
      • SRY related HMG box gene 2 antibody
      • SRY-box 2 antibody
      • Transcription factor SOX 2 antibody
      • Transcription factor SOX-2 antibody
      • ysb antibody
      see all

    Images

    • Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human fetal brain tissue labelling SOX2 with ab133337 at 1/50 dilution.
    • Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human glioma tissue labelling SOX2 with ab133337 at 1/50 dilution.
    • Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human brain tissue staining SOX2 with ab133337 at 1/50 dilution.
    • Anti-SOX2 antibody [EPR3130] (ab133337) at 1/500 dilution + NCCIT cell lysate at 10 µg

      Secondary
      Goat Anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 34 kDa
      Observed band size: 35 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Liu Z  et al. Specific marker expression and cell state of Schwann cells during culture in vitro. PLoS One 10:e0123278 (2015). WB, IF ; Mouse . Read more (PubMed: 25859851) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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