Overview

  • Product name
    Anti-SOX2 antibody [EPR3131]
    See all SOX2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3131] to SOX2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Sandwich ELISA, IHC-P, IHC - Wholemount, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Human, Leucoraja erinacea
  • Immunogen

    Synthetic peptide within Human SOX2. The exact sequence is proprietary.

  • Positive control
    • WB: NCCIT, F9, MCF-7 and C6 cell lysates; Human glioma lysate. IHC-P: Human gliocytoma, breast carcinoma, fetal stomach, fetal lung and embryonal carcinoma tissues; Sagittal maxillary incisor sections from E12 (A-D), E13 (E-H), E14 (I-L), and E15 (M-P) mouse embryos. ICC/IF: F9 and NCCIT cells; Mouse neuromesodermal progenitors. IHC-Wm: Leucoraja erinacea embryo; mouse blastocyst.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
Sandwich ELISA Use a concentration of 0.5 µg/ml.

For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Rabbit monoclonal [EPR3131] to SOX2 (ab92494) as Capture.

IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/60.

IHC - Wholemount Use at an assay dependent concentration.
ICC/IF 1/100.

For unpurified use at 1/60.

  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
    • Involvement in disease
      Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
    • Sequence similarities
      Contains 1 HMG box DNA-binding domain.
    • Post-translational
      modifications
      Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANOP3 antibody
      • cb236 antibody
      • Delta EF2a antibody
      • lcc antibody
      • MCOPS3 antibody
      • MGC148683 antibody
      • MGC2413 antibody
      • RGD1565646 antibody
      • Sex determining region Y box 2 antibody
      • SOX 2 antibody
      • Sox2 antibody
      • SOX2_HUMAN antibody
      • SRY (sex determining region Y) box 2 antibody
      • SRY box containing gene 2 antibody
      • SRY related HMG box 2 antibody
      • SRY related HMG box gene 2 antibody
      • SRY-box 2 antibody
      • Transcription factor SOX 2 antibody
      • Transcription factor SOX-2 antibody
      • ysb antibody
      see all

    Images

    • Transient Wnt and FGF signalling induce dual fated mouse neuromesodermal progenitors.

      Immunostaining of cells treated with FGF/Wnt revealed the coexpression of Brachyury with Sox2 (NMPs). In the absence of Wnt, NPCs express Sox2 but the expression of Brachyury is only evident in a very small proportion of cells.

    • SOX2 immunostaining in sagittal maxillary incisor sections from E12 (A-D), E13 (E-H), E14 (I-L), and E15 (M-P) embryos.

      At E13, strong SOX2 staining was seen in the lingual region of the epithelial dental lamina in all mice (E, G & H) except for the Usag-1+/+/Runx2-/- mice, in which SOX2 was found throughout the dental lamina (F). At E15, strong SOX2 staining was seen in the additional lingual bud in the Usag-1+/+/Runx2-/- mice (N).

    • Confocal image showing nuclear staining on F9 cells

      Ab92494 staining SOX2 in the F9 (mouse embryonal carcinoma) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG, Ab150077 (1/1000) was used as the secondary antibody. Counterstained with Ab7291 anti-Tubulin (1/1000), Ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

      Negative control 1 Ab92494 was used as the primary antibody at 1/200 and Ab150120 was used as the secondary at 1/1000.

      Negative control 2 Ab7291was used as the primary antibody at 1/1000 and Ab150077 was used as the secondary at 1/1000.

    • All lanes : Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution

      Lane 1 : NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate
      Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
      Lane 3 : SK-OV-3 (human ovarian cancer cell line) whole cell lysate
      Lane 4 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
      Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
      Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 7 : Human breast cancer tissue lysate
      Lane 8 : Human glioma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 34 kDa
      Observed band size: 34 kDa


      Exposure time: 3 minutes


      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Confocal image showing nuclear staining on NCCIT cells

      Ab92494 staining SOX2 in NCCIT cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG, Ab150077 (1/1000) was used as the secondary antibody. Counterstained with Ab7291 anti-Tubulin (1/1000), Ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

      Negative control 1 Ab92494 was used as the primary antibody at 1/200 and Ab150120 was used as the secondary at 1/1000.

      Negative control 2 Ab7291was used as the primary antibody at 1/1000 and Ab150077 was used as the secondary at 1/1000.

    • All lanes : Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution

      Lane 1 : F9 (mouse embryonic testicular cancer cell line) whole cell lysate
      Lane 2 : 4T1 (mouse mammary gland carcinoma cell line) whole cell lysate
      Lane 3 : Mouse hippocampus lysate
      Lane 4 : C6 (rat glial tumor cell line) whole cell lysate
      Lane 5 : Rat hippocampus lysate
      Lane 6 : Rat spinal cord lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 34 kDa
      Observed band size: 34 kDa


      Exposure time: 3 minutes


      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Confocal image showing negative staining on NIH/3T3 cells.

      Ab92494 staining SOX2 in the NIH/3T3 (mouse embryonic fibroblast cell line) (negative control) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG, ab150077 (1/1000) was used as the secondary antibody. Counterstained with ab7291 anti-Tubulin (1/1000), Ab150120 Alexa Fluor® 594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

      Negative control 1: ab92494 was used as the primary antibody at 1/200 and ab150120 was used as the secondary at 1/1000.

      Negative control 2: ab7291was used as the primary antibody at 1/1000 and ab150077 was used as the secondary at 1/1000.

    • Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution (unpurified) + NCCIT (human pluripotent embryonic carcinoma cell line) cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 34 kDa
      Observed band size: 34 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1500 dilution (purified) + F9 (mouse embryonic testicular cancer cell line) cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 34 kDa
      Observed band size: 34 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-SOX2 antibody [EPR3131] (ab92494) at 1/5000 dilution (unpurified)

      Lane 1 : NCCIT (human pluripotent embryonic carcinoma cell line) cell lysate
      Lane 2 : MCF-7 (human breast adenocarcinoma cell line) cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 34 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling SOX2 with unpurified ab92494 at 1/60. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling SOX2 with purified ab92494 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SOX2 with unpurified ab92494.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal stomach tissue labelling SOX2 with unpurified ab92494.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal lung tissue labelling SOX2 with unpurified ab92494.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human embryonal carcinoma tissue labelling SOX2 with unpurified ab92494.

    • Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human lung tissue. Unpurified ab92494 shows negative staining.

    • Negative control: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of negative human seminoma tissue using unpurified ab92494.

    • IHC - Wholemount analysis of Leucoraja erinacea embryo labelling SOX2 with unpurified ab92494 at 1/200. The sample was incubated with the primary antibody for 48 hours at 4°C in 10% fetal calf serum in PBT. Detection: DAB.

      See Abreview

    • IHC - Wholemount analysis of mouse blastocyst labelling SOX2 (pink) with unpurified ab92494 at 1/200. The sample was incubated with the primary antibody for 48 hours at 4°C. Nuclei stained with DAPI (grey).

      See Abreview

    • Standard Curve for SOX2 (Analyte: SOX2 protein (Human) (ab79950)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [57CT23.3.4] to SOX2 (ab75485) at 0.2µg/ml and Detector Antibody Rabbit monoclonal [EPR3131] to SOX2 (ab92494) at 0.5µg/ml.

    References

    This product has been referenced in:
    • Liu W  et al. Dioscin inhibits stem-cell-like properties and tumor growth of osteosarcoma through Akt/GSK3/ß-catenin signaling pathway. Cell Death Dis 9:343 (2018). Read more (PubMed: 29497056) »
    • Ji J  et al. XIAP Limits Autophagic Degradation of Sox2 and Is A Therapeutic Target in Nasopharyngeal Carcinoma Stem Cells. Theranostics 8:1494-1510 (2018). Read more (PubMed: 29556337) »
    See all 53 Publications for this product

    Customer reviews and Q&As

    1-10 of 15 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Neuromesodermal Progenitors)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    Neuromesodermal Progenitors
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Miss. Antigoni Gogolou

    Verified customer

    Submitted Nov 13 2018

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Xenopus laevis Recombinant protein (Clone expressed in Promega's TnT® SP6 High-Yield)
    Gel Running Conditions
    Non-reduced Denaturing (BioRad TGX Fast Cast 12%)
    Loading amount
    10 µg
    Specification
    Clone expressed in Promega's TnT® SP6 High-Yield
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted May 24 2018

    Application
    IHC - Wholemount
    Sample
    Mouse Embryo (Wholemount immunostaining of E7.75 mouse embryo)
    Specification
    Wholemount immunostaining of E7.75 mouse embryo

    Dr. Sophie Morgani

    Verified customer

    Submitted Mar 23 2018

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cow Tissue sections (Airway tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH 9.0
    Permeabilization
    No
    Specification
    Airway tissue
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Oct 21 2016

    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (4-12% bis tris gels)
    Loading amount
    100 µg
    Specification
    Heart
    Blocking step
    proprietary as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C

    Miss. Laxmi Vemalapally

    Verified customer

    Submitted Dec 22 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Glioma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer, pH 9
    Permeabilization
    Yes - Triton 0.025%
    Specification
    Glioma
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jun 29 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    • Endogenase peroxidase block with 3% H2O2 and DAKO S3022 antibody diluent (to reduce backgroun) as blocking agent for 5 minute(s) · Concentration: 100%
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako S1699
    Sample
    Human Tissue sections (patient brain tissues)
    Specification
    patient brain tissues
    Permeabilization
    No
    Fixative
    clinical samples (not sure)

    Abcam user community

    Verified customer

    Submitted Dec 04 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    IHC - Wholemount
    Sample
    Leucoraja erinacea Embryo (Whole embryo)
    Specification
    Whole embryo

    Dr. Andrew Gillis

    Verified customer

    Submitted Jul 24 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Glioblastoma primary cells)
    Loading amount
    30 µg
    Specification
    Glioblastoma primary cells
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Apr 16 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    IHC - Wholemount
    Sample
    Mouse Embryo (blastocyst)
    Specification
    blastocyst

    Abcam user community

    Verified customer

    Submitted Feb 06 2013

    1-10 of 15 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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