Name: Anti-SOX2 antibody [EPR3131]
Brand: RabMAb
SKU: ab92494
Rating: 5 (10 reviews)

Product name

Anti-SOX2 antibody [EPR3131]
See all SOX2 primary antibodies
Description

Rabbit monoclonal [EPR3131] to SOX2
Host species

Rabbit
Tested applications

Suitable for: WB, Sandwich ELISA, IHC-P, IHC - Wholemount, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivity

Reacts with: Mouse, Human, Leucoraja erinacea
Immunogen

Synthetic peptide within Human SOX2. The exact sequence is proprietary.
Positive control
- WB: NCCIT, F9, MCF-7 and C6 cell lysates; Human glioma lysate. IHC-P: Human gliocytoma, breast carcinoma, fetal stomach, fetal lung and embryonal carcinoma tissues; Sagittal maxillary incisor sections from E12 (A-D), E13 (E-H), E14 (I-L), and E15 (M-P) mouse embryos. ICC/IF: F9 and NCCIT cells; Mouse neuromesodermal progenitors. IHC-Wm: Leucoraja erinacea embryo; mouse blastocyst.
General notes

Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3131
Isotype

IgG
Research areas

Alternative Products
Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- MCF7 nuclear extract lysate (ab14860)
Related Products
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
- Recombinant Human SOX2 protein (ab79950)

Our Abpromise guarantee covers the use of ab92494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
Sandwich ELISA		Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Rabbit monoclonal [EPR3131] to SOX2 (ab92494) as Capture.
IHC-P		1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/60.
IHC - Wholemount		Use at an assay dependent concentration.
ICC/IF		1/100. For unpurified use at 1/60.

Application notes

Is unsuitable for Flow Cyt or IP.

Function

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
Involvement in disease

Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
Sequence similarities

Contains 1 HMG box DNA-binding domain.
Post-translational
modifications

Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
Cellular localization

Nucleus.
Target information above from: UniProt accession P48431 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6657 Human
- Entrez Gene: 20674 Mouse
- Omim: 184429 Human
- SwissProt: P48431 Human
- SwissProt: P48432 Mouse
- Unigene: 518438 Human
- Unigene: 65396 Mouse
Alternative names
- ANOP3 antibody
- cb236 antibody
- Delta EF2a antibody
- lcc antibody
- MCOPS3 antibody
- MGC148683 antibody
- MGC2413 antibody
- RGD1565646 antibody
- Sex determining region Y box 2 antibody
- SOX 2 antibody
- Sox2 antibody
- SOX2_HUMAN antibody
- SRY (sex determining region Y) box 2 antibody
- SRY box containing gene 2 antibody
- SRY related HMG box 2 antibody
- SRY related HMG box gene 2 antibody
- SRY-box 2 antibody
- Transcription factor SOX 2 antibody
- Transcription factor SOX-2 antibody
- ysb antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)Image from Gouti M et al., PLoS Biol. 2014;12(8):e1001937. Fig 2.; doi: 10.1371/journal.pbio.1001937.

Transient Wnt and FGF signalling induce dual fated mouse neuromesodermal progenitors.

Immunostaining of cells treated with FGF/Wnt revealed the coexpression of Brachyury with Sox2 (NMPs). In the absence of Wnt, NPCs express Sox2 but the expression of Brachyury is only evident in a very small proportion of cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)Image from Togo Y et al., PLoS One. 2016;11(8):e0161067. Fig 6.; doi: 10.1371/journal.pone.0161067.

SOX2 immunostaining in sagittal maxillary incisor sections from E12 (A-D), E13 (E-H), E14 (I-L), and E15 (M-P) embryos.

At E13, strong SOX2 staining was seen in the lingual region of the epithelial dental lamina in all mice (E, G & H) except for the Usag-1^+/+/Runx2^-/- mice, in which SOX2 was found throughout the dental lamina (F). At E15, strong SOX2 staining was seen in the additional lingual bud in the Usag-1^+/+/Runx2^-/- mice (N).
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)

Confocal image showing nuclear staining on F9 cells

Ab92494 staining SOX2 in the F9 (mouse embryonal carcinoma) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor^® 488-conjugated Goat anti-Rabbit IgG, Ab150077 (1/1000) was used as the secondary antibody. Counterstained with Ab7291 anti-Tubulin (1/1000), Ab150120 AlexaFluor^®594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

Negative control 1 Ab92494 was used as the primary antibody at 1/200 and Ab150120 was used as the secondary at 1/1000.

Negative control 2 Ab7291was used as the primary antibody at 1/1000 and Ab150077 was used as the secondary at 1/1000.
Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)

All lanes : Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution

Lane 1 : NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lane 3 : SK-OV-3 (human ovarian cancer cell line) whole cell lysate
Lane 4 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 7 : Human breast cancer tissue lysate
Lane 8 : Human glioma lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 34 kDa
Observed band size: 34 kDa

Exposure time: 3 minutes

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)

Confocal image showing nuclear staining on NCCIT cells

Ab92494 staining SOX2 in NCCIT cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor^® 488-conjugated Goat anti-Rabbit IgG, Ab150077 (1/1000) was used as the secondary antibody. Counterstained with Ab7291 anti-Tubulin (1/1000), Ab150120 AlexaFluor^®594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

Negative control 1 Ab92494 was used as the primary antibody at 1/200 and Ab150120 was used as the secondary at 1/1000.

Negative control 2 Ab7291was used as the primary antibody at 1/1000 and Ab150077 was used as the secondary at 1/1000.
Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)

All lanes : Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution

Lane 1 : F9 (mouse embryonic testicular cancer cell line) whole cell lysate
Lane 2 : 4T1 (mouse mammary gland carcinoma cell line) whole cell lysate
Lane 3 : Mouse hippocampus lysate
Lane 4 : C6 (rat glial tumor cell line) whole cell lysate
Lane 5 : Rat hippocampus lysate
Lane 6 : Rat spinal cord lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 34 kDa
Observed band size: 34 kDa

Exposure time: 3 minutes

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)

Confocal image showing negative staining on NIH/3T3 cells.

Ab92494 staining SOX2 in the NIH/3T3 (mouse embryonic fibroblast cell line) (negative control) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor^® 488-conjugated Goat anti-Rabbit IgG, ab150077 (1/1000) was used as the secondary antibody. Counterstained with ab7291 anti-Tubulin (1/1000), Ab150120 Alexa Fluor^® 594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

Negative control 1: ab92494 was used as the primary antibody at 1/200 and ab150120 was used as the secondary at 1/1000.

Negative control 2: ab7291was used as the primary antibody at 1/1000 and ab150077 was used as the secondary at 1/1000.
Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)

Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution (unpurified) + NCCIT (human pluripotent embryonic carcinoma cell line) cell lysate at 10 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 34 kDa
Observed band size: 34 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)

Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1500 dilution (purified) + F9 (mouse embryonic testicular cancer cell line) cell lysate at 10 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 34 kDa
Observed band size: 34 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)

All lanes : Anti-SOX2 antibody [EPR3131] (ab92494) at 1/5000 dilution (unpurified)

Lane 1 : NCCIT (human pluripotent embryonic carcinoma cell line) cell lysate
Lane 2 : MCF-7 (human breast adenocarcinoma cell line) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling SOX2 with unpurified ab92494 at 1/60. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling SOX2 with purified ab92494 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SOX2 with unpurified ab92494.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal stomach tissue labelling SOX2 with unpurified ab92494.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal lung tissue labelling SOX2 with unpurified ab92494.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human embryonal carcinoma tissue labelling SOX2 with unpurified ab92494.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human lung tissue. Unpurified ab92494 shows negative staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

Negative control: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of negative human seminoma tissue using unpurified ab92494.
IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (ab92494)Image courtesy of Dr. Gillis, Dalhousie University, Canada.

IHC - Wholemount analysis of Leucoraja erinacea embryo labelling SOX2 with unpurified ab92494 at 1/200. The sample was incubated with the primary antibody for 48 hours at 4°C in 10% fetal calf serum in PBT. Detection: DAB.
See Abreview
IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (ab92494)This image is courtesy of an anonymous Abreview.

IHC - Wholemount analysis of mouse blastocyst labelling SOX2 (pink) with unpurified ab92494 at 1/200. The sample was incubated with the primary antibody for 48 hours at 4°C. Nuclei stained with DAPI (grey).
See Abreview
Sandwich ELISA - Anti-SOX2 antibody [EPR3131] (ab92494)

Standard Curve for SOX2 (Analyte: SOX2 protein (Human) (ab79950)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [57CT23.3.4] to SOX2 (ab75485) at 0.2µg/ml and Detector Antibody Rabbit monoclonal [EPR3131] to SOX2 (ab92494) at 0.5µg/ml.

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols
Protocol Booklet

This product has been referenced in:

Chopra S et al. Cancer Stem Cells, CD44, and Outcomes Following Chemoradiation in Locally Advanced Cervical Cancer: Results From a Prospective Study. Int J Radiat Oncol Biol Phys 103:161-168 (2019). Read more (PubMed: 30213750) »
Pang Y et al. MYC and DNMT3A-mediated DNA methylation represses microRNA-200b in triple negative breast cancer. J Cell Mol Med 22:6262-6274 (2018). Read more (PubMed: 30324719) »

See all 56 Publications for this product

Publishing research using ab92494? Please let us know so that we can cite the reference in this datasheet.

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1-10 of 10 Abreviews

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Neuromesodermal Progenitors)

Permeabilization

Yes - 0.5% Triton X-100

Specification

Neuromesodermal Progenitors

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Paraformaldehyde

Miss. Antigoni Gogolou

Verified customer

Submitted Nov 13 2018

Abcam has not validated the combination of species/application used in this Abreview.

Application

Western blot

Sample

Xenopus laevis Recombinant protein (Clone expressed in Promega's TnT® SP6 High-Yield)

Gel Running Conditions

Non-reduced Denaturing (BioRad TGX Fast Cast 12%)

Loading amount

10 µg

Specification

Clone expressed in Promega's TnT® SP6 High-Yield

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted May 24 2018

Application

IHC - Wholemount

Sample

Mouse Embryo (Wholemount immunostaining of E7.75 mouse embryo)

Specification

Wholemount immunostaining of E7.75 mouse embryo

Dr. Sophie Morgani

Verified customer

Submitted Mar 23 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Cow Tissue sections (Airway tissue)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH 9.0

Permeabilization

Specification

Airway tissue

Blocking step

Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Oct 21 2016

Application

Western blot

Sample

Pig Tissue lysate - whole (Heart)

Gel Running Conditions

Reduced Denaturing (4-12% bis tris gels)

Loading amount

100 µg

Specification

Heart

Blocking step

proprietary as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C

Miss. Laxmi Vemalapally

Verified customer

Submitted Dec 22 2015

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Glioma)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer, pH 9

Permeabilization

Yes - Triton 0.025%

Specification

Glioma

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 29 2015

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

• Endogenase peroxidase block with 3% H2O2 and DAKO S3022 antibody diluent (to reduce backgroun) as blocking agent for 5 minute(s) · Concentration: 100%

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Dako S1699

Sample

Human Tissue sections (patient brain tissues)

Specification

patient brain tissues

Permeabilization

Fixative

clinical samples (not sure)

Abcam user community

Verified customer

Submitted Dec 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

IHC - Wholemount

Sample

Leucoraja erinacea Embryo (Whole embryo)

Specification

Whole embryo

Dr. Andrew Gillis

Verified customer

Submitted Jul 24 2013

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Human Cell lysate - whole cell (Glioblastoma primary cells)

Loading amount

30 µg

Specification

Glioblastoma primary cells

Gel Running Conditions

Reduced Denaturing (10%)

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

IHC - Wholemount

Sample

Mouse Embryo (blastocyst)

Specification

blastocyst

Abcam user community

Verified customer

Submitted Feb 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-SOX2 antibody [EPR3131] (ab92494)

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