Recombinant
RabMAb

Recombinant Anti-SOX2 antibody [EPR3131] (ab92494)

Reviews (17)Q&A (5)References (81)

Overview

  • Product name

    Anti-SOX2 antibody [EPR3131]
    See all SOX2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3131] to SOX2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Sandwich ELISA, IHC-P, IHC - Wholemount, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Human, Leucoraja erinacea

  • Immunogen

    Synthetic peptide within Human SOX2. The exact sequence is proprietary.
    Database link: P48431

  • Positive control

    • WB: NCCIT, F9, MCF-7 and C6 cell lysates; Human glioma lysate. IHC-P: Human gliocytoma, breast carcinoma, fetal stomach, fetal lung and embryonal carcinoma tissues; Sagittal maxillary incisor sections from E12, E13, E14, and E15 mouse embryos. ICC/IF: F9 and NCCIT cells; Mouse neuromesodermal progenitors. IHC-Wm: Leucoraja erinacea embryo; mouse blastocyst.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab92494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
Sandwich ELISA Use a concentration of 0.5 µg/ml.

For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Rabbit monoclonal [EPR3131] to SOX2 (ab92494) as Capture.
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/60.
IHC - Wholemount Use at an assay dependent concentration.
ICC/IF 1/100.

For unpurified use at 1/60.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.

    • Involvement in disease

      Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.

    • Sequence similarities

      Contains 1 HMG box DNA-binding domain.

    • Post-translational
      modifications

      Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • ANOP3 antibody
        • cb236 antibody
        • Delta EF2a antibody
        • lcc antibody
        • MCOPS3 antibody
        • MGC148683 antibody
        • MGC2413 antibody
        • RGD1565646 antibody
        • Sex determining region Y box 2 antibody
        • SOX 2 antibody
        • Sox2 antibody
        • SOX2_HUMAN antibody
        • SRY (sex determining region Y) box 2 antibody
        • SRY box containing gene 2 antibody
        • SRY related HMG box 2 antibody
        • SRY related HMG box gene 2 antibody
        • SRY-box 2 antibody
        • Transcription factor SOX 2 antibody
        • Transcription factor SOX-2 antibody
        • ysb antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)
        Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)Image from Gouti M et al., PLoS Biol. 2014;12(8):e1001937. Fig 2.; doi: 10.1371/journal.pbio.1001937. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

        Transient Wnt and FGF signalling induce dual fated mouse neuromesodermal progenitors.

        Immunostaining of cells treated with FGF/Wnt revealed the coexpression of Brachyury with Sox2 (NMPs). In the absence of Wnt, NPCs express Sox2 but the expression of Brachyury is only evident in a very small proportion of cells.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)Image from Togo Y et al., PLoS One. 2016;11(8):e0161067. Fig 6.; doi: 10.1371/journal.pone.0161067. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

        SOX2 immunostaining in sagittal maxillary incisor sections from E12 (A-D), E13 (E-H), E14 (I-L), and E15 (M-P) embryos.

        At E13, strong SOX2 staining was seen in the lingual region of the epithelial dental lamina in all mice (E, G & H) except for the Usag-1+/+/Runx2-/- mice, in which SOX2 was found throughout the dental lamina (F). At E15, strong SOX2 staining was seen in the additional lingual bud in the Usag-1+/+/Runx2-/- mice (N).

      • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)
        Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)

        Confocal image showing nuclear staining on F9 cells

        Ab92494 staining SOX2 in the F9 (mouse embryonal carcinoma) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG, Ab150077 (1/1000) was used as the secondary antibody. Counterstained with Ab7291 anti-Tubulin (1/1000), Ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

        Negative control 1 Ab92494 was used as the primary antibody at 1/200 and Ab150120 was used as the secondary at 1/1000.

        Negative control 2 Ab7291was used as the primary antibody at 1/1000 and Ab150077 was used as the secondary at 1/1000.

      • Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
        Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
        All lanes : Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution

        Lane 1 : NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate
        Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
        Lane 3 : SK-OV-3 (human ovarian cancer cell line) whole cell lysate
        Lane 4 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
        Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
        Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
        Lane 7 : Human breast cancer tissue lysate
        Lane 8 : Human glioma lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 34 kDa
        Observed band size: 34 kDa


        Exposure time: 3 minutes


        Blocking buffer: 5% NFDM/TBST
        Dilution buffer: 5% NFDM/TBST

      • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)
        Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)

        Confocal image showing nuclear staining on NCCIT cells

        Ab92494 staining SOX2 in NCCIT cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG, Ab150077 (1/1000) was used as the secondary antibody. Counterstained with Ab7291 anti-Tubulin (1/1000), Ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

        Negative control 1 Ab92494 was used as the primary antibody at 1/200 and Ab150120 was used as the secondary at 1/1000.

        Negative control 2 Ab7291was used as the primary antibody at 1/1000 and Ab150077 was used as the secondary at 1/1000.

      • Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
        Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
        All lanes : Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution

        Lane 1 : F9 (mouse embryonic testicular cancer cell line) whole cell lysate
        Lane 2 : 4T1 (mouse mammary gland carcinoma cell line) whole cell lysate
        Lane 3 : Mouse hippocampus lysate
        Lane 4 : C6 (rat glial tumor cell line) whole cell lysate
        Lane 5 : Rat hippocampus lysate
        Lane 6 : Rat spinal cord lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 34 kDa
        Observed band size: 34 kDa


        Exposure time: 3 minutes


        Blocking buffer: 5% NFDM/TBST
        Dilution buffer: 5% NFDM/TBST

      • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)
        Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)

        Confocal image showing negative staining on NIH/3T3 cells.

        Ab92494 staining SOX2 in the NIH/3T3 (mouse embryonic fibroblast cell line) (negative control) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG, ab150077 (1/1000) was used as the secondary antibody. Counterstained with ab7291 anti-Tubulin (1/1000), Ab150120 Alexa Fluor® 594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

        Negative control 1: ab92494 was used as the primary antibody at 1/200 and ab150120 was used as the secondary at 1/1000.

        Negative control 2: ab7291was used as the primary antibody at 1/1000 and ab150077 was used as the secondary at 1/1000.

      • Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
        Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
        Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution (unpurified) + NCCIT (human pluripotent embryonic carcinoma cell line) cell lysate at 10 µg

        Secondary
        Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

        Predicted band size: 34 kDa
        Observed band size: 34 kDa



        Blocking buffer and concentration: 5% NFDM/TBST.

        Diluting buffer and concentration: 5% NFDM /TBST.

      • Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
        Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
        Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1500 dilution (purified) + F9 (mouse embryonic testicular cancer cell line) cell lysate at 10 µg

        Secondary
        Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

        Predicted band size: 34 kDa
        Observed band size: 34 kDa



        Blocking buffer and concentration: 5% NFDM/TBST.

        Diluting buffer and concentration: 5% NFDM /TBST.

      • Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
        Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
        All lanes : Anti-SOX2 antibody [EPR3131] (ab92494) at 1/5000 dilution (unpurified)

        Lane 1 : NCCIT (human pluripotent embryonic carcinoma cell line) cell lysate
        Lane 2 : MCF-7 (human breast adenocarcinoma cell line) cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

        Predicted band size: 34 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling SOX2 with unpurified ab92494 at 1/60. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling SOX2 with purified ab92494 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SOX2 with unpurified ab92494.

        Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal stomach tissue labelling SOX2 with unpurified ab92494.

        Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal lung tissue labelling SOX2 with unpurified ab92494.

        Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human embryonal carcinoma tissue labelling SOX2 with unpurified ab92494.

        Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

        Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human lung tissue. Unpurified ab92494 shows negative staining.

        Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

        Negative control: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of negative human seminoma tissue using unpurified ab92494.

        Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

      • IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (ab92494)
        IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (ab92494)Image courtesy of Dr. Gillis, Dalhousie University, Canada.

        IHC - Wholemount analysis of Leucoraja erinacea embryo labelling SOX2 with unpurified ab92494 at 1/200. The sample was incubated with the primary antibody for 48 hours at 4°C in 10% fetal calf serum in PBT. Detection: DAB.

        See Abreview

      • IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (ab92494)
        IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (ab92494)This image is courtesy of an anonymous Abreview.

        IHC - Wholemount analysis of mouse blastocyst labelling SOX2 (pink) with unpurified ab92494 at 1/200. The sample was incubated with the primary antibody for 48 hours at 4°C. Nuclei stained with DAPI (grey).

        See Abreview

      • Sandwich ELISA - Anti-SOX2 antibody [EPR3131] (ab92494)
        Sandwich ELISA - Anti-SOX2 antibody [EPR3131] (ab92494)

        Standard Curve for SOX2 (Analyte: SOX2 protein (Human) (ab79950)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [57CT23.3.4] to SOX2 (ab75485) at 0.2µg/ml and Detector Antibody Rabbit monoclonal [EPR3131] to SOX2 (ab92494) at 0.5µg/ml.

      References

      This product has been referenced in:

      • Chopra S  et al. Cancer Stem Cells, CD44, and Outcomes Following Chemoradiation in Locally Advanced Cervical Cancer: Results From a Prospective Study. Int J Radiat Oncol Biol Phys 103:161-168 (2019). Read more (PubMed: 30213750) »
      • Wymeersch FJ  et al. Transcriptionally dynamic progenitor populations organised around a stable niche drive axial patterning. Development 146:N/A (2019). Read more (PubMed: 30559277) »
      See all 81 Publications for this product

      Publishing research using ab92494? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-10 of 17 Abreviews

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX2 antibody [EPR3131]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Rat Tissue sections (Cerebellum)
      Antigen retrieval step
      None
      Permeabilization
      Yes - PBS 1x, Triton x-100 1%
      Specification
      Cerebellum
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 20 2020

      Western blot abreview for Anti-SOX2 antibody [EPR3131]

       Below Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HEK293)
      Gel Running Conditions
      Reduced Denaturing (4-15% gradient)
      Loading amount
      20 µg
      Specification
      HEK293
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 27 2020

      Western blot abreview for Anti-SOX2 antibody [EPR3131]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Brain)
      Gel Running Conditions
      Reduced Denaturing (4-15% gradient)
      Loading amount
      40 µg
      Specification
      Brain
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 26 2020

      Western blot abreview for Anti-SOX2 antibody [EPR3131]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Cell lysate - whole cell (Hippocampal)
      Gel Running Conditions
      Reduced Denaturing (4-15% gradient)
      Loading amount
      40 µg
      Specification
      Hippocampal
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 26 2020

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX2 antibody [EPR3131]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Mouse Tissue sections (Brain (Thalamus))
      Antigen retrieval step
      None
      Permeabilization
      Yes - PBS 1x, Triton x-100 1%
      Specification
      Brain (Thalamus)
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 26 2020

      Western blot abreview for Anti-SOX2 antibody [EPR3131]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (DU145 Prostate cancer cell line)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      20 µg
      Specification
      DU145 Prostate cancer cell line
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 10 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-SOX2 antibody [EPR3131]

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (hPSC differentiated)
      Permeabilization
      Yes - Triton 0.5%
      Specification
      hPSC differentiated
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Ms. Ingrid Saldana

      Verified customer

      Submitted Jul 31 2019

      Immunocytochemistry/ Immunofluorescence abreview for Anti-SOX2 antibody [EPR3131]

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Neuromesodermal Progenitors)
      Permeabilization
      Yes - 0.5% Triton X-100
      Specification
      Neuromesodermal Progenitors
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Miss. Antigoni Gogolou

      Verified customer

      Submitted Nov 13 2018

      Western blot abreview for Anti-SOX2 antibody [EPR3131]

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Xenopus laevis Recombinant protein (Clone expressed in Promega's TnT® SP6 High-Yield)
      Gel Running Conditions
      Non-reduced Denaturing (BioRad TGX Fast Cast 12%)
      Loading amount
      10 µg
      Specification
      Clone expressed in Promega's TnT® SP6 High-Yield
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More

      Abcam user community

      Verified customer

      Submitted May 24 2018

      IHC - Wholemount abreview for Anti-SOX2 antibody [EPR3131]

       Excellent
      Abreviews
      abreview image
      Application
      IHC - Wholemount
      Sample
      Mouse Embryo (Wholemount immunostaining of E7.75 mouse embryo)
      Specification
      Wholemount immunostaining of E7.75 mouse embryo
      Read More

      Dr. Sophie Morgani

      Verified customer

      Submitted Mar 23 2018

      1-10 of 17 Abreviews

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