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RecombinantRabMAb

Recombinant Anti-SOX2 antibody [EPR3131] (ab92494)

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Reviews (22)Q&A (5)References (139)

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Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
  • IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (ab92494)
  • IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Sandwich ELISA - Anti-SOX2 antibody [EPR3131] (ab92494)
  • Anti-SOX2 antibody [EPR3131] (ab92494)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3131] to SOX2
  • Suitable for: WB, IHC - Wholemount, Sandwich ELISA, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human, Leucoraja erinacea

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

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Overview

  • Product name

    Anti-SOX2 antibody [EPR3131]
    See all SOX2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3131] to SOX2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC - Wholemount, Sandwich ELISA, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Leucoraja erinacea
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NCCIT, F9, MCF-7 and C6 cell lysates; Human glioma lysate. IHC-P: Human gliocytoma, breast carcinoma, fetal stomach, fetal lung and embryonal carcinoma tissues; Sagittal maxillary incisor sections from E12, E13, E14, and E15 mouse embryos. ICC/IF: F9 and NCCIT cells; Mouse neuromesodermal progenitors. IHC-Wm: Leucoraja erinacea embryo; mouse blastocyst.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3131
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Leucine Zipper
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HMG Box
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Stem Cells
    • Germline Stem Cells
    • Embryonic Germ Cells
    • Developmental Biology
    • Reproduction
    • Germ cell markers
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-SOX2 antibody [EPR3131] (ab195358)
    • Alexa Fluor® 647 Anti-SOX2 antibody [EPR3131] (ab196637)
    • Anti-SOX2 antibody [EPR3131] - BSA and Azide free (ab215970)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 nuclear extract lysate (ab14860)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Recombinant Human SOX2 protein (ab79950)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (8)
1/1000 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
IHC - Wholemount (3)
Use at an assay dependent concentration.
Sandwich ELISA
Use a concentration of 0.5 µg/ml.

For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Rabbit monoclonal [EPR3131] to SOX2 (ab92494) as Capture.

IHC-P (3)
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/60.

ICC/IF (3)
1/100.
Notes
WB
1/1000 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
IHC - Wholemount
Use at an assay dependent concentration.
Sandwich ELISA
Use a concentration of 0.5 µg/ml.

For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Rabbit monoclonal [EPR3131] to SOX2 (ab92494) as Capture.

IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/60.

ICC/IF
1/100.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • Involvement in disease

    Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Post-translational
    modifications

    Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P48431 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6657 Human
    • Entrez Gene: 20674 Mouse
    • Entrez Gene: 499593 Rat
    • Omim: 184429 Human
    • SwissProt: P48431 Human
    • SwissProt: P48432 Mouse
    • Unigene: 518438 Human
    • Unigene: 65396 Mouse
    • Alternative names

      • ANOP3 antibody
      • cb236 antibody
      • Delta EF2a antibody
      • lcc antibody
      • MCOPS3 antibody
      • MGC148683 antibody
      • MGC2413 antibody
      • RGD1565646 antibody
      • Sex determining region Y box 2 antibody
      • SOX 2 antibody
      • Sox2 antibody
      • SOX2_HUMAN antibody
      • SRY (sex determining region Y) box 2 antibody
      • SRY box containing gene 2 antibody
      • SRY related HMG box 2 antibody
      • SRY related HMG box gene 2 antibody
      • SRY-box 2 antibody
      • Transcription factor SOX 2 antibody
      • Transcription factor SOX-2 antibody
      • ysb antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)
      Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)

      Confocal image showing nuclear staining on F9 cells

      Ab92494 staining SOX2 in the F9 (mouse embryonal carcinoma) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG, Ab150077 (1/1000) was used as the secondary antibody. Counterstained with Ab7291 anti-Tubulin (1/1000), Ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

      Negative control 1 Ab92494 was used as the primary antibody at 1/200 and Ab150120 was used as the secondary at 1/1000.

      Negative control 2 Ab7291was used as the primary antibody at 1/1000 and Ab150077 was used as the secondary at 1/1000.

    • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)
      Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)

      ab92494 staining SOX2 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab92494 at 1/100 dilution and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)
      Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)Image from Gouti M et al., PLoS Biol. 2014;12(8):e1001937. Fig 2.; doi: 10.1371/journal.pbio.1001937. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Transient Wnt and FGF signalling induce dual fated mouse neuromesodermal progenitors.

      Immunostaining of cells treated with FGF/Wnt revealed the coexpression of Brachyury with Sox2 (NMPs). In the absence of Wnt, NPCs express Sox2 but the expression of Brachyury is only evident in a very small proportion of cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)Image from Togo Y et al., PLoS One. 2016;11(8):e0161067. Fig 6.; doi: 10.1371/journal.pone.0161067. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      SOX2 immunostaining in sagittal maxillary incisor sections from E12 (A-D), E13 (E-H), E14 (I-L), and E15 (M-P) embryos.

      At E13, strong SOX2 staining was seen in the lingual region of the epithelial dental lamina in all mice (E, G & H) except for the Usag-1+/+/Runx2-/- mice, in which SOX2 was found throughout the dental lamina (F). At E15, strong SOX2 staining was seen in the additional lingual bud in the Usag-1+/+/Runx2-/- mice (N).

    • Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
      Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
      All lanes : Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution

      Lane 1 : NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate
      Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
      Lane 3 : SK-OV-3 (human ovarian cancer cell line) whole cell lysate
      Lane 4 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
      Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
      Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 7 : Human breast cancer tissue lysate
      Lane 8 : Human glioma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 34 kDa
      Observed band size: 34 kDa


      Exposure time: 3 minutes


      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)
      Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)

      Confocal image showing nuclear staining on NCCIT cells

      Ab92494 staining SOX2 in NCCIT cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG, Ab150077 (1/1000) was used as the secondary antibody. Counterstained with Ab7291 anti-Tubulin (1/1000), Ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

      Negative control 1 Ab92494 was used as the primary antibody at 1/200 and Ab150120 was used as the secondary at 1/1000.

      Negative control 2 Ab7291was used as the primary antibody at 1/1000 and Ab150077 was used as the secondary at 1/1000.

    • Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
      Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
      All lanes : Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution

      Lane 1 : F9 (mouse embryonic testicular cancer cell line) whole cell lysate
      Lane 2 : 4T1 (mouse mammary gland carcinoma cell line) whole cell lysate
      Lane 3 : Mouse hippocampus lysate
      Lane 4 : C6 (rat glial tumor cell line) whole cell lysate
      Lane 5 : Rat hippocampus lysate
      Lane 6 : Rat spinal cord lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 34 kDa
      Observed band size: 34 kDa


      Exposure time: 3 minutes


      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)
      Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)

      Confocal image showing negative staining on NIH/3T3 cells.

      Ab92494 staining SOX2 in the NIH/3T3 (mouse embryonic fibroblast cell line) (negative control) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG, ab150077 (1/1000) was used as the secondary antibody. Counterstained with ab7291 anti-Tubulin (1/1000), Ab150120 Alexa Fluor® 594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

      Negative control 1: ab92494 was used as the primary antibody at 1/200 and ab150120 was used as the secondary at 1/1000.

      Negative control 2: ab7291was used as the primary antibody at 1/1000 and ab150077 was used as the secondary at 1/1000.

    • Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
      Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
      Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution (unpurified) + NCCIT (human pluripotent embryonic carcinoma cell line) cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 34 kDa
      Observed band size: 34 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
      Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
      Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1500 dilution (purified) + F9 (mouse embryonic testicular cancer cell line) cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 34 kDa
      Observed band size: 34 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
      Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)
      All lanes : Anti-SOX2 antibody [EPR3131] (ab92494) at 1/5000 dilution (unpurified)

      Lane 1 : NCCIT (human pluripotent embryonic carcinoma cell line) cell lysate
      Lane 2 : MCF-7 (human breast adenocarcinoma cell line) cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 34 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling SOX2 with unpurified ab92494 at 1/60. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling SOX2 with purified ab92494 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SOX2 with unpurified ab92494.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal stomach tissue labelling SOX2 with unpurified ab92494.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal lung tissue labelling SOX2 with unpurified ab92494.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human embryonal carcinoma tissue labelling SOX2 with unpurified ab92494.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

      Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human lung tissue. Unpurified ab92494 shows negative staining.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

      Negative control: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of negative human seminoma tissue using unpurified ab92494.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

    • IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (ab92494)
      IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (ab92494)Image courtesy of Dr. Gillis, Dalhousie University, Canada.

      IHC - Wholemount analysis of Leucoraja erinacea embryo labelling SOX2 with unpurified ab92494 at 1/200. The sample was incubated with the primary antibody for 48 hours at 4°C in 10% fetal calf serum in PBT. Detection: DAB.

      See Abreview

    • IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (ab92494)
      IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (ab92494)This image is courtesy of an anonymous Abreview.

      IHC - Wholemount analysis of mouse blastocyst labelling SOX2 (pink) with unpurified ab92494 at 1/200. The sample was incubated with the primary antibody for 48 hours at 4°C. Nuclei stained with DAPI (grey).

      See Abreview

    • Sandwich ELISA - Anti-SOX2 antibody [EPR3131] (ab92494)
      Sandwich ELISA - Anti-SOX2 antibody [EPR3131] (ab92494)

      Standard Curve for SOX2 (Analyte: SOX2 protein (Human) (ab79950)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [57CT23.3.4] to SOX2 (ab75485) at 0.2µg/ml and Detector Antibody Rabbit monoclonal [EPR3131] to SOX2 (ab92494) at 0.5µg/ml.

    • Anti-SOX2 antibody [EPR3131] (ab92494)
      Anti-SOX2 antibody [EPR3131] (ab92494)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols
    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (139)

    Publishing research using ab92494? Please let us know so that we can cite the reference in this datasheet.

    ab92494 has been referenced in 139 publications.

    • Zhong Y & Lan J Overexpression of Eukaryotic translation initiation factor 3D induces stem cell-like properties and metastasis in cervix cancer by activating FAK through inhibiting degradation of GRP78. Bioengineered 13:1952-1961 (2022). Human . PubMed: 35104170
    • Xie H  et al. Exosome-transmitted circVMP1 facilitates the progression and cisplatin resistance of non-small cell lung cancer by targeting miR-524-5p-METTL3/SOX2 axis. Drug Deliv 29:1257-1271 (2022). WB ; Human . PubMed: 35467477
    • Hernández-Ainsa C  et al. Development and characterization of cell models harbouring mtDNA deletions for in vitro study of Pearson syndrome. Dis Model Mech 15:N/A (2022). PubMed: 35191981
    • Arrieta VA  et al. ERK1/2 phosphorylation predicts survival following anti-PD-1 immunotherapy in recurrent glioblastoma. Nat Cancer 2:1372-1386 (2021). PubMed: 35121903
    • Wang Z  et al. Overexpression of Fgf8 in the epidermis inhibits hair follicle development. Exp Dermatol 30:494-502 (2021). PubMed: 33141445
    View all Publications for this product

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    1-10 of 22 Abreviews

    Western blot abreview for Anti-SOX2 antibody [EPR3131]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Oesophagus)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    20 µg
    Specification
    Oesophagus
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Helena Santos-Rosa

    Verified customer

    Submitted Oct 11 2022

    Immunocytochemistry abreview for Anti-SOX2 antibody [EPR3131]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human (Embryonic stem cells)
    Permeabilization
    Yes - methanol
    Specification
    Embryonic stem cells
    Blocking step
    Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 21 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SOX2 antibody [EPR3131]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Pig Cell (embryo)
    Permeabilization
    Yes - tritonx-100
    Specification
    embryo
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 23 2021

    Immunohistochemistry free floating abreview for Anti-SOX2 antibody [EPR3131]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 26 2021

    Immunohistochemistry free floating abreview for Anti-SOX2 antibody [EPR3131]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Rat Tissue sections (Cerebellum)
    Specification
    Cerebellum
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 26 2021

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX2 antibody [EPR3131]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Cerebellum)
    Antigen retrieval step
    None
    Permeabilization
    Yes - PBS 1x, Triton x-100 1%
    Specification
    Cerebellum
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 20 2020

    Western blot abreview for Anti-SOX2 antibody [EPR3131]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Gel Running Conditions
    Reduced Denaturing (4-15% gradient)
    Loading amount
    20 µg
    Specification
    HEK293
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 27 2020

    Western blot abreview for Anti-SOX2 antibody [EPR3131]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (4-15% gradient)
    Loading amount
    40 µg
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 26 2020

    Western blot abreview for Anti-SOX2 antibody [EPR3131]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Hippocampal)
    Gel Running Conditions
    Reduced Denaturing (4-15% gradient)
    Loading amount
    40 µg
    Specification
    Hippocampal
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 26 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX2 antibody [EPR3131]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain (Thalamus))
    Antigen retrieval step
    None
    Permeabilization
    Yes - PBS 1x, Triton x-100 1%
    Specification
    Brain (Thalamus)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 26 2020

    1-10 of 22 Abreviews

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