Anti-SOX2 (GlcNAc S248) antibody (ab136167)
Key features and details
- Rabbit polyclonal to SOX2 (GlcNAc S248)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-SOX2 (GlcNAc S248) antibody
See all SOX2 primary antibodies -
Description
Rabbit polyclonal to SOX2 (GlcNAc S248) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in E14Tg2A , MCF7 whole cell lysates and IOUD2 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab136167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa). |
Target
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Function
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. -
Involvement in disease
Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Post-translational
modificationsSumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 20674 Mouse
- SwissProt: P48432 Mouse
- Unigene: 65396 Mouse
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Alternative names
- ANOP3 antibody
- cb236 antibody
- Delta EF2a antibody
see all
Images
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All lanes : Anti-SOX2 (GlcNAc S248) antibody (ab136167) at 1 µg/ml
Lane 1 : MCF7 whole cell lysate
Lane 2 : Mouse embryonic stem cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 34 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesGel type: MOPS
Blocking buffer: 2% BSA block
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All lanes : Anti-SOX2 (GlcNAc S248) antibody (ab136167) at 1 µg/ml
Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 2 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 3 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate with SOX2 (glcnac S248) peptide (ab157602) at 1 µg/ml
Lane 4 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate with SOX2 (glcnac S248) peptide (ab157602) at 1 µg/ml
Lane 5 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate with Mouse SOX2 peptide (ab157771) at 1 µg/ml
Lane 6 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate with Mouse SOX2 peptide (ab157771) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 148 kDa, 24 kDa, 29 kDa, 62 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab136167 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab136167 has been referenced in 1 publication.
- Speakman CM et al. Elevated O-GlcNAc levels activate epigenetically repressed genes and delay mouse ES cell differentiation without affecting naïve to primed cell transition. Stem Cells N/A:N/A (2014). WB ; Mouse . PubMed: 24898611