Overview

  • Product name

  • Description

    Rabbit polyclonal to SOX21
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: IHC-Fr
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Chicken, Human, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human SOX21 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab26974)

Properties

Applications

Our Abpromise guarantee covers the use of ab24604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IHC-Fr.
  • Target

    Images

    • Immunohistochemistry (Formaldehyde/PFA-fixed paraffin-embedded sections) using SOX21 antibody (ab24604)on Rat Tissue sections (adult brain lateral ventricle subventricular zone). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT°C. ab24604 used at 1/300 incubated for 16 hours. Secondary Antibody: biotin conjugated anti rabbit IgG (1/300). NB: Sox21 protein expression showin in this image is in concurrs with published mRNA positivity in the subventricular zone and rostro-migratory stream in the adult rat.

    References

    ab24604 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (embryonic brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.01M Sodium-citrate buffer, pH 6.0
    Permeabilization
    Yes - 0.3% Triton X-100 in PBS for 10 minutes
    Specification
    embryonic brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative
    Paraformaldehyde

    Dr. Laura Lahti

    Verified customer

    Submitted Jun 03 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (adult brain lateral ventricle svz)
    Specification
    adult brain lateral ventricle svz
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citric acid pH6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

    Mr. Carl Hobbs

    Verified customer

    Submitted Nov 05 2007

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (E12.5 Whole brain section)
    Specification
    E12.5 Whole brain section
    Fixative
    Paraformaldehyde
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

    Dr. Dean Griffiths

    Verified customer

    Submitted Aug 21 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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