Key features and details
- Mouse monoclonal [CL4688] to SOX21
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2a
Product nameAnti-SOX21 antibody [CL4688]
See all SOX21 primary antibodies
DescriptionMouse monoclonal [CL4688] to SOX21
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chicken, Xenopus laevis, Zebrafish
- WB; NTERA-2 cell extract. IHC-P: Mouse embryo E12, Mouse forebrain and cerebral cortex tissue. Human stomach and cervix tissue. ICC/IF: PC-3 cells
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab220024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Predicted molecular weight: 29 kDa.|
|ICC/IF||Use a concentration of 2 - 10 µg/ml.|
FunctionMay play a role as an activator of transcription of OPRM1.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
- Information by UniProt
- SOX A antibody
- SOX-A antibody
- sox21 antibody
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling SOX21 with ab220024 at 1/200 dilution.
Anti-SOX21 antibody [CL4688] (ab220024) at 1/500 dilution + NTERA-2 cell extract.
Predicted band size: 29 kDa
Immunocytochemistry/ Immunofluorescence anaysis of PC-3 cells labeling SOX21 with ab220024 at 2-10 µg/mL. Cells were fixed with PFA and permeabilised with Triton X-100. The data is showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemical analysis of paraffin-embedded Mouse embryo E14 tissue labeling SOX21 with ab220024 at 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse forebrain tissue labeling SOX21 with ab220024 at 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling SOX21 with ab220024 at 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded Human cervix tissue labeling SOX21 with ab220024 at 1/200 dilution.
ab220024 has not yet been referenced specifically in any publications.