Anti-SOX21 antibody [CL4688] (ab220024)
Key features and details
- Mouse monoclonal [CL4688] to SOX21
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-SOX21 antibody [CL4688]
See all SOX21 primary antibodies -
Description
Mouse monoclonal [CL4688] to SOX21 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken, Xenopus laevis, Zebrafish -
Immunogen
Synthetic peptide corresponding to Human SOX21 aa 250 to the C-terminus.
Database link: Q9Y651 -
Positive control
- WB; NTERA-2 cell extract. IHC-P: Mouse embryo E12, Mouse forebrain and cerebral cortex tissue. Human stomach and cervix tissue. ICC/IF: PC-3 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL4688 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/500 - 1/1000. Predicted molecular weight: 29 kDa.
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ICC/IF |
Use a concentration of 2 - 10 µg/ml.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Predicted molecular weight: 29 kDa. |
ICC/IF
Use a concentration of 2 - 10 µg/ml. |
Target
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Function
May play a role as an activator of transcription of OPRM1. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 107051027 Chicken
- Entrez Gene: 11166 Human
- Entrez Gene: 223227 Mouse
- Entrez Gene: 100337612 Xenopus laevis
- Omim: 604974 Human
- SwissProt: Q9W7R5 Chicken
- SwissProt: Q9Y651 Human
- SwissProt: Q811W0 Mouse
see all -
Alternative names
- SOX A antibody
- SOX-A antibody
- sox21 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling SOX21 with ab220024 at 1/200 dilution.
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Anti-SOX21 antibody [CL4688] (ab220024) at 1/500 dilution + NTERA-2 cell extract.
Predicted band size: 29 kDa -
Immunocytochemistry/ Immunofluorescence anaysis of PC-3 cells labeling SOX21 with ab220024 at 2-10 µg/mL. Cells were fixed with PFA and permeabilised with Triton X-100. The data is showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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Immunohistochemical analysis of paraffin-embedded Mouse embryo E14 tissue labeling SOX21 with ab220024 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse forebrain tissue labeling SOX21 with ab220024 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling SOX21 with ab220024 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human cervix tissue labeling SOX21 with ab220024 at 1/200 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab220024 has not yet been referenced specifically in any publications.