Overview

  • Product name

  • Description

    Rabbit polyclonal to SOX3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC - Wholemountmore details
  • Species reactivity

    Reacts with: Zebrafish
  • Immunogen

    Recombinant fragment within Zebrafish SOX3 (internal sequence). The exact sequence is proprietary.
    Database link: Q6EJB7

  • Positive control

    • IHC-Wm: 1 day-post-fertilization zebrafish embryo. WB: Whole zebrafish extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab229629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.
IHC - Wholemount 1/100 - 1/500.

Target

  • Function

    Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells.
  • Involvement in disease

    Defects in SOX3 are a cause of panhypopituitarism X-linked (PHPX) [MIM:312000]. Affected individuals have absent infundibulum, anterior pituitary hypoplasia, and ectopic posterior pituitary.
    Defects in SOX3 are the cause of mental retardation X-linked with isolated growth hormone deficiency (MRXGH) [MIM:300123].
    Defects in SOX3 are the cause of 46,XX sex reversal type 3 (SRXX3) [MIM:300833]. A condition in which male gonads develop in a genetic female (female to male sex reversal). Note=Copy number variations (CNV) encompassing or in close proximity to SOX3 are responsible for XX male reversal. These variations include two duplications of approximately 123 kb and 85 kb, the former of which spans the entire SOX3 gene; a 343 kb deletion immediately upstream of SOX3 that is probably responsible of altered regulation (and not increased dosage) of SOX3; a large (approximately 6 Mb) duplication that encompasses SOX3 and at least 18 additional distally located genes. Its proximal breakpoint falls within the SOX3 regulatory region. This large rearrangement has been found in a patient with XX male reversal and a complex phenotype that also includes a scrotal hypoplasia, microcephaly, developmental delay, and growth retardation.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

    • Alternative names

      • GHDX antibody
      • MRGH antibody
      • PHP antibody
      • PHPX antibody
      • sox3 antibody
      • SOX3_HUMAN antibody
      • SOXB antibody
      • SRY (sex determining region Y)-box 3 antibody
      • SRY Box 3 antibody
      • SRY-related HMG-box gene 3 antibody
      • Transcription factor Sox-3 antibody
      see all

    Images

    • Anti-SOX3 antibody (ab229629) at 1/1000 dilution + Whole zebrafish extract at 30 µg

      Developed using the ECL technique.

      Predicted band size: 33 kDa



      12% SDS-PAGE

    • 1 day-post-fertilization zebrafish embryo stained for SOX3 with ab229629 at a 1/100 dilution in immunohistochemical analysis.

    • 1 day-post-fertilization zebrafish embryo stained for SOX3 with ab229629 at a 1/100 dilution in immunohistochemical analysis.

    References

    ab229629 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab229629.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up