Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab42471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionTranscription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells.
Involvement in diseaseDefects in SOX3 are a cause of panhypopituitarism X-linked (PHPX) [MIM:312000]. Affected individuals have absent infundibulum, anterior pituitary hypoplasia, and ectopic posterior pituitary.
Defects in SOX3 are the cause of mental retardation X-linked with isolated growth hormone deficiency (MRXGH) [MIM:300123].
Defects in SOX3 are the cause of 46,XX sex reversal type 3 (SRXX3) [MIM:300833]. A condition in which male gonads develop in a genetic female (female to male sex reversal). Note=Copy number variations (CNV) encompassing or in close proximity to SOX3 are responsible for XX male reversal. These variations include two duplications of approximately 123 kb and 85 kb, the former of which spans the entire SOX3 gene; a 343 kb deletion immediately upstream of SOX3 that is probably responsible of altered regulation (and not increased dosage) of SOX3; a large (approximately 6 Mb) duplication that encompasses SOX3 and at least 18 additional distally located genes. Its proximal breakpoint falls within the SOX3 regulatory region. This large rearrangement has been found in a patient with XX male reversal and a complex phenotype that also includes a scrotal hypoplasia, microcephaly, developmental delay, and growth retardation.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
- Information by UniProt
- GHDX antibody
- MRGH antibody
- PHP antibody
Anti-SOX3 antibody (ab42471) at 0.5 µg/ml + Raji cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Diluted in 5% skim milk / PBS buffer, gel concentration 12%
Western blot procedure primary murine neural stem cell cultures
Proteins denaturized at 95oC for 10 min Run on 11% PAGE, and then transferred overnight to a nitrocellulose membrane
Blocked in 5% non fat dried milk in PBST, 1 hr, RT shaking
Ab42471 1:2000 dilution in 5% Marvel in PBST) , 1 hr, RT shaking
Wash 3 time in PBST 10 min, RT shaking
HRP anti-Rabbit (1:5000 dilution in 5% Milk in PBST),1 hr, RT shaking
Wash 3 times in PBST 10 min, RT shaking
incubate in ECL-Plus and exposed to film
Data submitted courtesy of: Yu-Huan Shih
This product has been referenced in:
- Wang G et al. Upregulation of mtSSB by interleukin-6 promotes cell growth through mitochondrial biogenesis-mediated telomerase activation in colorectal cancer. Int J Cancer N/A:N/A (2018). Read more (PubMed: 30415472) »
- Corsinotti A et al. Distinct SoxB1 networks are required for naïve and primed pluripotency. Elife 6:N/A (2017). Read more (PubMed: 29256862) »