Key features and details
- Mouse monoclonal [CL4725] to SOX3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-SOX3 antibody [CL4725]
See all SOX3 primary antibodies
DescriptionMouse monoclonal [CL4725] to SOX3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
EpitopeBinds to an epitope located within the peptide sequence LISLPFPPDS as determined by overlapping synthetic peptides.
- WB: NTERA-2 cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab242366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa.|
FunctionTranscription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells.
Involvement in diseaseDefects in SOX3 are a cause of panhypopituitarism X-linked (PHPX) [MIM:312000]. Affected individuals have absent infundibulum, anterior pituitary hypoplasia, and ectopic posterior pituitary.
Defects in SOX3 are the cause of mental retardation X-linked with isolated growth hormone deficiency (MRXGH) [MIM:300123].
Defects in SOX3 are the cause of 46,XX sex reversal type 3 (SRXX3) [MIM:300833]. A condition in which male gonads develop in a genetic female (female to male sex reversal). Note=Copy number variations (CNV) encompassing or in close proximity to SOX3 are responsible for XX male reversal. These variations include two duplications of approximately 123 kb and 85 kb, the former of which spans the entire SOX3 gene; a 343 kb deletion immediately upstream of SOX3 that is probably responsible of altered regulation (and not increased dosage) of SOX3; a large (approximately 6 Mb) duplication that encompasses SOX3 and at least 18 additional distally located genes. Its proximal breakpoint falls within the SOX3 regulatory region. This large rearrangement has been found in a patient with XX male reversal and a complex phenotype that also includes a scrotal hypoplasia, microcephaly, developmental delay, and growth retardation.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
- Information by UniProt
- GHDX antibody
- MRGH antibody
- PHP antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab242366 has not yet been referenced specifically in any publications.