Overview

  • Product name

    Anti-SOX4 antibody [154C4a]
    See all SOX4 primary antibodies
  • Description

    Mouse monoclonal [154C4a] to SOX4
  • Host species

    Mouse
  • Tested applications

    Suitable for: ChIP, WB, Dot blotmore details
  • Species reactivity

    Reacts with: Mouse, Recombinant fragment
  • Immunogen

    Recombinant fragment of human SOX4 from an internal sequence.

  • Positive control

    • Recombinant human SOX4.

Properties

Applications

Our Abpromise guarantee covers the use of ab70598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 21878674
WB Use at an assay dependent dilution. Detects a band of approximately 38 kDa (predicted molecular weight: 47 kDa).
Dot blot Use at an assay dependent dilution.

Target

  • Function

    Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif.
  • Tissue specificity

    Testis, brain, and heart.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ecotropic viral integration site 16 antibody
    • EVI 16 antibody
    • EVI16 antibody
    • Sox4 antibody
    • SOX4_HUMAN antibody
    • SRY (sex determining region Y) box 4 antibody
    • SRY box containing gene 4 antibody
    • SRY related HMG box gene 4 antibody
    • Transcription factor SOX-4 antibody
    see all

Images

  • Anti-SOX4 antibody [154C4a] (ab70598) + immunising recombinant protein

    Predicted band size: 47 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?



    The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.

References

This product has been referenced in:

  • Lee H  et al. TMEM2 Is a SOX4-Regulated Gene That Mediates Metastatic Migration and Invasion in Breast Cancer. Cancer Res 76:4994-5005 (2016). Read more (PubMed: 27328729) »
  • Aue G  et al. Sox4 cooperates with PU.1 haploinsufficiency in murine myeloid leukemia. Blood 118:4674-81 (2011). ChIP ; Mouse . Read more (PubMed: 21878674) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (human normal keratinocytes and SCC cancer cell lin)
Specification
human normal keratinocytes and SCC cancer cell lin
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 13 2014

Answer

Thank you for your enquiry. I am pleased to hear this customer would like to make use of our testing discount offer and test ab70598SOX4 antibody [154C4a] in human samples.

I can provide the following code:

DISCOUNT CODE: ####
Expiration date: ####

This code will give you: 1 freeprimary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab70598 in human samplesand include this code in the Additional Comments section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receivingthe Abreview and wishthe customerluck withtheir research.

Read More

Answer

Thank you for your phone call. I am sorry to hear that ab80261 did not work as expected. Unfortunately, since the antibody was purchased over 1 year ago and our guarantee is valid for 6 months after the purchase, I am not able to offer a free of charge replacement, credit or refund in this case. However, I am happy to send you a testing discount code for trying ab70598 in human samples. DISCOUNT CODE: xxx Expiration date: xxx I am very pleased to hear you would like to accept our offer and test ab70598 in human. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for human and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (cancer cells)
Loading amount
30 µg
Specification
cancer cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 27 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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