Overview

  • Product name
    Anti-SOX4 antibody [154C4a]
    See all SOX4 primary antibodies
  • Description
    Mouse monoclonal [154C4a] to SOX4
  • Host species
    Mouse
  • Tested applications
    Suitable for: ChIP, WB, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Recombinant fragment
  • Immunogen

    Recombinant fragment of human SOX4 from an internal sequence.

  • Positive control
    • Recombinant human SOX4.

Properties

Applications

Our Abpromise guarantee covers the use of ab70598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 21878674
WB Use at an assay dependent dilution. Detects a band of approximately 38 kDa (predicted molecular weight: 47 kDa).
Dot blot Use at an assay dependent dilution.

Target

  • Function
    Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif.
  • Tissue specificity
    Testis, brain, and heart.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ecotropic viral integration site 16 antibody
    • EVI 16 antibody
    • EVI16 antibody
    • Sox4 antibody
    • SOX4_HUMAN antibody
    • SRY (sex determining region Y) box 4 antibody
    • SRY box containing gene 4 antibody
    • SRY related HMG box gene 4 antibody
    • Transcription factor SOX-4 antibody
    see all

Images

  • Anti-SOX4 antibody [154C4a] (ab70598) + immunising recombinant protein

    Predicted band size: 47 kDa
    Observed band size: 38 kDa (why is the actual band size different from the predicted?)



    The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.

References

This product has been referenced in:
  • Lee H  et al. TMEM2 Is a SOX4-Regulated Gene That Mediates Metastatic Migration and Invasion in Breast Cancer. Cancer Res 76:4994-5005 (2016). Read more (PubMed: 27328729) »
  • Aue G  et al. Sox4 cooperates with PU.1 haploinsufficiency in murine myeloid leukemia. Blood 118:4674-81 (2011). ChIP ; Mouse . Read more (PubMed: 21878674) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (human normal keratinocytes and SCC cancer cell lin)
Specification
human normal keratinocytes and SCC cancer cell lin
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 13 2014

Thank you for your enquiry. I am pleased to hear this customer would like to make use of our testing discount offer and test ab70598SOX4 antibody [154C4a] in human samples.

I can provide the following code:

DISCOUNT CODE: #### Read More

Thank you for your phone call. I am sorry to hear that ab80261 did not work as expected. Unfortunately, since the antibody was purchased over 1 year ago and our guarantee is valid for 6 months after the purchase, I am not able to offer a free of cha...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (cancer cells)
Loading amount
30 µg
Specification
cancer cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 27 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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