Overview

  • Product name

  • Description

    Rabbit polyclonal to SOX4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human SOX4 (N terminal). Immunogen located within the region from aa 1 and 50.
    Database link: Q06945
    (Peptide available as ab138332)

  • Positive control

    • Human fetal kidney lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-SOX4 antibody (ab90696) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal kidney lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 47 kDa



    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling SOX4 with ab90696.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization
No
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 22 2018

Question
Answer

Thank you for your reply and I am sorry that we were not able to resolve the SOX4 western blotting results.

Your credit note ID is ***.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Question
Answer

Thank you for your reply and for providing that extra information.

Based on the results that you provided, I think that the bands at about 44kDa is specific but I am still not sure what the higher molecular weight band could be. The fact that it is being blocked by the peptide, does suggest it is specific though.

I think the best way forward is to either to refund the cost of your original order. Please let me know if this is satisfactory to you and I will then proceed.

I look forward to your reply.

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Answer

Thank you for your reply.

Because of the inconsistent results you are gtting with different SOX4 antibodies, I am sending you the peptide for ab90696, free of charge, so you can use it in a blocking experiment, to prove the specificity of the signal that you are getting with ab96096. The peptide is on back order but you should have it in a week or so.

If you still have issue after using the peptide in the blocking experiment, then please let me know.

Good luck and hope you have a great weekend.

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