Anti-SOX5 antibody (ab94396)
Key features and details
- Rabbit polyclonal to SOX5
- Suitable for: IHC-Fr, ChIP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SOX5 antibody -
Description
Rabbit polyclonal to SOX5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, ChIP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken, Cow, Pig, Zebrafish
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
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| ChIP |
Use at an assay dependent concentration. PubMed: 22547066
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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ChIP
Use at an assay dependent concentration. PubMed: 22547066 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa). |
Target
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Function
Binds specifically to the DNA sequence 5'-AACAAT-3'. Activates transcription of COL2A1 and AGC1 in vitro. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6660 Human
- Entrez Gene: 20678 Mouse
- Omim: 604975 Human
- SwissProt: P35711 Human
- SwissProt: P35710 Mouse
- Unigene: 657542 Human
- Unigene: 1752 Mouse
- Unigene: 482428 Mouse
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Alternative names
- L SOX5 antibody
- MGC35153 antibody
- Sex determining region Y box 5 antibody
see all
Images
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Western blot - Anti-SOX5 antibody (ab94396)Anti-SOX5 antibody (ab94396) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Additional bands at: 24 kDa, 28 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab94396 has been referenced in 20 publications.
- Tian Y et al. SOX-5 activates a novel ROR?t enhancer to facilitate experimental autoimmune encephalomyelitis by promoting Th17 cell differentiation. Nat Commun 12:481 (2021). PubMed: 33473108
- Kitajima K et al. Light-induced local gene expression in primary chick cell culture system. Dev Growth Differ 63:189-198 (2021). PubMed: 33733477
- Feng Y et al. A novel lncRNA SOX2OT promotes the malignancy of human colorectal cancer by interacting with miR-194-5p/SOX5 axis. Cell Death Dis 12:499 (2021). PubMed: 33993197
- Chen M et al. Transactivation of SOX5 by Brachyury promotes breast cancer bone metastasis. Carcinogenesis 41:551-560 (2020). PubMed: 31713604
- Zawerton A et al. Widening of the genetic and clinical spectrum of Lamb-Shaffer syndrome, a neurodevelopmental disorder due to SOX5 haploinsufficiency. Genet Med 22:524-537 (2020). PubMed: 31578471