Anti-SOX5 antibody (ab94396)
Key features and details
- Rabbit polyclonal to SOX5
- Suitable for: IHC-Fr, ChIP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SOX5 antibody -
Description
Rabbit polyclonal to SOX5 -
Host species
Rabbit -
Specificity
Replenishment batches of ab94396 are tested in WB. Previous batches were additionally validated in ChIP and IHC-Fr. These applications are still expected to work and are covered by our Abpromise guarantee.
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Tested applications
Suitable for: IHC-Fr, ChIP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken, Cow, Pig, Zebrafish -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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ChIP |
Use at an assay dependent concentration. PubMed: 22547066
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
ChIP
Use at an assay dependent concentration. PubMed: 22547066 |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa). |
Target
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Function
Binds specifically to the DNA sequence 5'-AACAAT-3'. Activates transcription of COL2A1 and AGC1 in vitro. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6660 Human
- Entrez Gene: 20678 Mouse
- Omim: 604975 Human
- SwissProt: P35711 Human
- SwissProt: P35710 Mouse
- Unigene: 657542 Human
- Unigene: 1752 Mouse
- Unigene: 482428 Mouse
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Alternative names
- L SOX5 antibody
- MGC35153 antibody
- Sex determining region Y box 5 antibody
see all
Images
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Anti-SOX5 antibody (ab94396) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Additional bands at: 24 kDa, 28 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (29)
ab94396 has been referenced in 29 publications.
- Junaković A et al. Laminar dynamics of deep projection neurons and mode of subplate formation are hallmarks of histogenetic subdivisions of the human cingulate cortex before onset of arealization. Brain Struct Funct 228:613-633 (2023). PubMed: 36592215
- Shi L et al. CircSEC24A promotes IL-1β-induced apoptosis and inflammation in chondrocytes by regulating miR-142-5p/SOX5 axis. Biotechnol Appl Biochem 69:701-713 (2022). PubMed: 33751650
- Harb K et al. Pum2 and TDP-43 refine area-specific cytoarchitecture post-mitotically and modulate translation of Sox5, Bcl11b, and Rorb mRNAs in developing mouse neocortex. Elife 11:N/A (2022). PubMed: 35262486
- Dilly GA et al. Cell-type specific changes in PKC-delta neurons of the central amygdala during alcohol withdrawal. Transl Psychiatry 12:289 (2022). PubMed: 35859068
- Liang Q et al. circ-LDLRAD3 Knockdown Reduces Cisplatin Chemoresistance and Inhibits the Development of Gastric Cancer with Cisplatin Resistance through miR-588 Enrichment-Mediated SOX5 Inhibition. Gut Liver N/A:N/A (2022). PubMed: 35975639