Key features and details
- Rabbit polyclonal to SOX6
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SOX6 antibody
See all SOX6 primary antibodies
DescriptionRabbit polyclonal to SOX6
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region within amino acids 778-828 of Human SOX6 (EAW68458.1).
- 293T, HeLa and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab125196 was affinity purified using an epitope specific to SOX6 immobilized on a solid support.
Our Abpromise guarantee covers the use of ab125196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 92 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionTranscriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.
Tissue specificityExpressed in a wide variety of tissues, most abundantly in skeletal muscle.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
modificationsSumoylation inhibits the transcriptional activity.
- Information by UniProt
- HSSOX 6 antibody
- HSSOX6 antibody
- SOX 6 antibody
All lanes : Anti-SOX6 antibody (ab125196) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 92 kDa
Exposure time: 3 minutes
Detection of SOX6 by Western Blot of Immunprecipitate.
ab125196 at 1µg/ml staining SOX6 in HeLa whole cell lysate immunoprecipitated using ab125196 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab125196 has been referenced in 1 publication.
- Leow SC et al. The transcription factor SOX6 contributes to the developmental origins of obesity by promoting adipogenesis. Development 143:950-61 (2016). PubMed: 26893351