Overview

  • Product name

    Anti-SOX6 antibody - ChIP Grade
    See all SOX6 primary antibodies
  • Description

    Rabbit polyclonal to SOX6 - ChIP Grade
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WB, IHC - Wholemount, ChIP, IHC-FoFr, CHIPseqmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Mouse SOX6.

    Read Abcam's proprietary immunogen policy (Peptide available as ab30530.)

  • Positive control

    • IHC (PFA-fixed): on perfusion fixed wildtype P18 mouse dentate gyrus.

Properties

Applications

Our Abpromise guarantee covers the use of ab30455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Antigen retrieval is not essential but may optimise staining.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC - Wholemount Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 21985497
IHC-FoFr 1/300.
CHIPseq Use at an assay dependent concentration. PubMed: 21985497

Target

  • Function

    Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.
  • Tissue specificity

    Expressed in a wide variety of tissues, most abundantly in skeletal muscle.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Post-translational
    modifications

    Sumoylation inhibits the transcriptional activity.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • HSSOX 6 antibody
    • HSSOX6 antibody
    • SOX 6 antibody
    • sox6 antibody
    • SOX6_HUMAN antibody
    • SOXD antibody
    • SRY (sex determining region Y) box 6 antibody
    • SRY box 6 antibody
    • SRY box containing gene 6 antibody
    • Transcription factor SOX 6 antibody
    • Transcription factor Sox-6 antibody
    • Transcription factor SOX6 antibody
    see all

Images

  • IHC (PFA-fixed) image of SOX6 (ab30455) on sections of P18 mouse dentate gyrus. The mice were perfusion fixed with 4% PFA and then post-fixed in 4% PFA for a few hours. The blocks were prepared in agarose/sucrose and cut using a vibratome (50 µm). The sections were permeabilized with 1% Triton X-100 for 30 mins and blocked with 10% FBS for 2 hours at room temperature, then incubated overnight at 4°C with the primary antibody. The primary antibody was used at a 1/100 dilution and detected with donkey anti-rabbit 488 secondary at 1/1000 dilution.

  • ab30455 staining Sox6 in post-natal mouse brain sections. PFA-fixed post-natal mouse brain sections were incubated for 30 minutes in blocking solution followed by an overnight incubation with ab30455 (1/500) at 4°C. No staining was observed in sections obtained from Sox6 knockout mice.
  • IHC-FoFr image of Sox6 staining in Rat hippocampus sections using ab30455.The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. immunostainings were performed using the ‘free floating’ technique.

    See Abreview

References

This product has been referenced in:

  • Simmons SC  et al. Determination of circuit-specific morphological adaptations in ventral tegmental area dopamine neurons by chronic morphine. Mol Brain 12:10 (2019). Read more (PubMed: 30736837) »
  • Takimoto A  et al. Differential transactivation of the upstream aggrecan enhancer regulated by PAX1/9 depends on SOX9-driven transactivation. Sci Rep 9:4605 (2019). Read more (PubMed: 30872687) »
See all 29 Publications for this product

Customer reviews and Q&As

21-21 of 21 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain (hippocampus))
Specification
Brain (hippocampus)

Dr. Sophie Pezet

Verified customer

Submitted Nov 23 2010

21-21 of 21 Abreviews or Q&A

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