Overview

  • Product name

    Anti-SOX6 antibody - ChIP Grade
    See all SOX6 primary antibodies
  • Description

    Rabbit polyclonal to SOX6 - ChIP Grade
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WB, IHC - Wholemount, ChIP, IHC-FoFr, CHIPseqmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Mouse SOX6.

    Read Abcam's proprietary immunogen policy (Peptide available as ab30530.)

  • Positive control

    • IHC (PFA-fixed): on perfusion fixed wildtype P18 mouse dentate gyrus.

Properties

Applications

Our Abpromise guarantee covers the use of ab30455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Antigen retrieval is not essential but may optimise staining.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC - Wholemount Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 21985497
IHC-FoFr 1/300.
CHIPseq Use at an assay dependent concentration. PubMed: 21985497

Target

  • Function

    Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.
  • Tissue specificity

    Expressed in a wide variety of tissues, most abundantly in skeletal muscle.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Post-translational
    modifications

    Sumoylation inhibits the transcriptional activity.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • HSSOX 6 antibody
    • HSSOX6 antibody
    • SOX 6 antibody
    • sox6 antibody
    • SOX6_HUMAN antibody
    • SOXD antibody
    • SRY (sex determining region Y) box 6 antibody
    • SRY box 6 antibody
    • SRY box containing gene 6 antibody
    • Transcription factor SOX 6 antibody
    • Transcription factor Sox-6 antibody
    • Transcription factor SOX6 antibody
    see all

Images

  • IHC (PFA-fixed) image of SOX6 (ab30455) on sections of P18 mouse dentate gyrus. The mice were perfusion fixed with 4% PFA and then post-fixed in 4% PFA for a few hours. The blocks were prepared in agarose/sucrose and cut using a vibratome (50 µm). The sections were permeabilized with 1% Triton X-100 for 30 mins and blocked with 10% FBS for 2 hours at room temperature, then incubated overnight at 4°C with the primary antibody. The primary antibody was used at a 1/100 dilution and detected with donkey anti-rabbit 488 secondary at 1/1000 dilution.

  • ab30455 staining Sox6 in post-natal mouse brain sections. PFA-fixed post-natal mouse brain sections were incubated for 30 minutes in blocking solution followed by an overnight incubation with ab30455 (1/500) at 4°C. No staining was observed in sections obtained from Sox6 knockout mice.
  • IHC-FoFr image of Sox6 staining in Rat hippocampus sections using ab30455.The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. immunostainings were performed using the ‘free floating’ technique.

    See Abreview

References

This product has been referenced in:

  • Simmons SC  et al. Determination of circuit-specific morphological adaptations in ventral tegmental area dopamine neurons by chronic morphine. Mol Brain 12:10 (2019). Read more (PubMed: 30736837) »
  • Takimoto A  et al. Differential transactivation of the upstream aggrecan enhancer regulated by PAX1/9 depends on SOX9-driven transactivation. Sci Rep 9:4605 (2019). Read more (PubMed: 30872687) »
See all 29 Publications for this product

Customer reviews and Q&As

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1-10 of 15 Abreviews

Application
Western blot
Sample
Rabbit Tissue lysate - whole (brain)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
0.2 µg
Treatment
hypoxic 40 minutes at E25
Specification
brain

Abcam user community

Verified customer

Submitted Jan 25 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse cortical astrocytes)
Specification
mouse cortical astrocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Radhika Menon

Verified customer

Submitted Sep 06 2017

Application
Western blot
Sample
Rabbit Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
brain
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 03 2016

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Mouse Tissue lysate - whole (Mouse cortex)
Specification
Mouse cortex
Total protein in input
20 µg

Abcam user community

Verified customer

Submitted Dec 31 2014

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (7.5%)
Sample
Human Tissue lysate - whole (HEK293T cells)
Specification
HEK293T cells
Treatment
Myc-tagged Sox6 co-transfected with E1, E2 or E3 ligases
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Oct 24 2014

Abreviews
Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Tissue lysate - nuclear (Primary chondrocytes)
Specification
Primary chondrocytes
Negative control
DNA primers targeting non-binding region
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
DNA primers targeting promoter region

Abcam user community

Verified customer

Submitted Oct 23 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (7.5%)
Sample
Mouse Cell lysate - whole cell (Muscle C2C12 cells)
Specification
Muscle C2C12 cells
Treatment
100 μg/ml cycloheximide (CHX)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Oct 23 2014

Application
IHC - Wholemount
Sample
Mouse Tissue (Mouse limb muscle)
Specification
Mouse limb muscle

Abcam user community

Verified customer

Submitted May 02 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Mouse Cell (NIH 3T3 fibroblasts)
Specification
NIH 3T3 fibroblasts
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 02 2014

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Sample
Mouse Tissue sections (Mouse epidermis)
Specification
Mouse epidermis
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 02 2014

1-10 of 15 Abreviews

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