The antibody produced (diluted 1:300) a beautiful nuclear staining in the adult cortex and hippocampus. The picture loaded shows the staining obtained at the level of the hippocampus.
The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
Dr. Sophie Pezet
Submitted Nov 23 2010
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