Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX6 antibody - ChIP Grade

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain (hippocampus))
Specification
Brain (hippocampus)

Other product details

Dilution
1/300
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBST

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
The antibody produced (diluted 1:300) a beautiful nuclear staining in the adult cortex and hippocampus. The picture loaded shows the staining obtained at the level of the hippocampus.

The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

Dr. Sophie Pezet

Verified customer

Submitted Nov 23 2010

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