Western blot abreview for Anti-SOX6 antibody

Excellent
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (7.5%)
Sample
Mouse Cell lysate - whole cell (Muscle C2C12 cells)
Specification
Muscle C2C12 cells
Treatment
100 μg/ml cycloheximide (CHX)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 0.1% Tween 20 + 5% BSA
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Bands
Specific: 75~100 kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
24hr treatment by 100 ´g/ml protein synthesis inhibitor cycloheximide (CHX), the protein level is reduced by around half.

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Submitted Oct 23 2014

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