C-terminal Myc-tagged Sox6 was co-transfected with E1, E2 or E3 ligases. Cells were harvested 24 post-transfection, lysed, immunoprecipitated with myc tag antibody, and analyzed by western blot. The membranes were bloted with both myc antibody (with longer exposure) and Sox6 antibody (Abcam ab30455, with 1 min exposure).
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Submitted Oct 24 2014
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