Western blot abreview for Anti-SOX6 antibody

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Abreviews
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (7.5%)
Sample
Human Tissue lysate - whole (HEK293T cells)
Specification
HEK293T cells
Treatment
Myc-tagged Sox6 co-transfected with E1, E2 or E3 ligases
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 0.1% Tween 20 + 5% BSA
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: 100 kDa
Exposure
1 minute(s) and 0 second(s)

Additional data

Additional Notes
C-terminal Myc-tagged Sox6 was co-transfected with E1, E2 or E3 ligases. Cells were harvested 24 post-transfection, lysed, immunoprecipitated with myc tag antibody, and analyzed by western blot. The membranes were bloted with both myc antibody (with longer exposure) and Sox6 antibody (Abcam ab30455, with 1 min exposure).

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Submitted Oct 24 2014

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