Overview

  • Product name

    Anti-SOX6 antibody [CL5690]
    See all SOX6 primary antibodies
  • Description

    Mouse monoclonal [CL5690] to SOX6
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human SOX6 aa 349-367.
    Sequence:

    NLDTFEHGGGHSYNHKQIE


    Database link: P35712

  • Positive control

    • IHC-P: Human glioma, renal cancer and testis tissue. ICC/IF: WM-115 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab243576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.
  • Tissue specificity

    Expressed in a wide variety of tissues, most abundantly in skeletal muscle.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Post-translational
    modifications

    Sumoylation inhibits the transcriptional activity.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • HSSOX 6 antibody
    • HSSOX6 antibody
    • SOX 6 antibody
    • sox6 antibody
    • SOX6_HUMAN antibody
    • SOXD antibody
    • SRY (sex determining region Y) box 6 antibody
    • SRY box 6 antibody
    • SRY box containing gene 6 antibody
    • Transcription factor SOX 6 antibody
    • Transcription factor Sox-6 antibody
    • Transcription factor SOX6 antibody
    see all

Images

  •  
     
    Immunocytochemistry/immunofluorescence analysis of ab243576 of CACO-2 cells using SOX6 with ab243576 (green).Microtubule nuclear probes are visualised in red. 
  • Formalin-fixed, paraffin-embedded human glioma tissue stained for SOX6 with ab243576 at a 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human testis tissue stained for SOX6 with ab243576 at a 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human renal cancer tissue stained for SOX6 with ab243576 at a 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for SOX6 with ab243576 at a 1/200 dilution in immunohistochemical analysis. No nuclear immunoreactivity in lymphoid cells (negative control).

References

ab243576 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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