Overview

  • Product name

  • Description

    Rabbit polyclonal to SOX8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human SOX8 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab125935)

  • Positive control

    • This antibody gave a positive signal in both Human Testis and Human Colon tissue lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).

Target

Images

  • All lanes : Anti-SOX8 antibody (ab104245) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes

References

ab104245 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
ChIP
Sample
Human Cell lysate - nuclear (Sarcoma cancer cells expressing SOX8)
Negative control
Heterochromatic gene desert
Specification
Sarcoma cancer cells expressing SOX8
Detection step
Semiquantitative PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 12 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde in DMEM
Positive control
Active Super Enhancer site

Berkley Gryder

Verified customer

Submitted Dec 20 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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