Overview

  • Product name
    Anti-SOX9 antibody [3C10]
    See all SOX9 primary antibodies
  • Description
    Mouse monoclonal [3C10] to SOX9
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: EQLSPSHYSE QQQHSPQQIA YSPFNLPHYS PSYPPITRSQ YDYTDHQNSS SYYSHAAGQG TGLYSTFTYM NPAQRPMYTP IADTSGVPSI PQTHSPQHWE QPVYTQLTRP, corresponding to amino acids 400-509 of human SOX9 (NP_000337) with a 26 kDa tag

  • Positive control
    • WB: HepG2 whole cell lysate (ab7900). IHC-P: Human tonsil tissue. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 22/03/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Applications

Our Abpromise guarantee covers the use of ab76997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
ELISA Use at an assay dependent concentration. Detection limit for ab76997 is approximately 30 ng/ml when used as a capture antibody.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.

Target

  • Function
    Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
  • Involvement in disease
    Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • campomelic dysplasia autosomal sex reversal antibody
    • CMD 1 antibody
    • CMD1 antibody
    • CMPD 1 antibody
    • CMPD1 antibody
    • SOX 9 antibody
    • Sox9 antibody
    • SOX9_HUMAN antibody
    • SRA 1 antibody
    • SRA1 antibody
    • SRXX2 antibody
    • SRXY10 antibody
    • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
    • SRY (sex determining region Y) box 9 antibody
    • SRY (sex determining region Y)-box 9 antibody
    • SRY (sex-determining region Y)-box 9 protein antibody
    • SRY related HMG box gene 9 antibody
    • Transcription factor SOX 9 antibody
    • Transcription factor SOX-9 antibody
    • transcription factor SOX9 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling SOX9 with ab76997 at 0.7 µg/ml.

    This image was generated using the ascites version of the product.

     

  • Immunocytochemistry/Immunofluorescence analysis of SOX9 expression in HepG2 cells, using 10 µg/ml of ab76997.

    This image was generated using the ascites version of the product.

     

  • Overlay histogram showing HepG2 cells stained with ab76997 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76997, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated using the ascites version of the product.

     

  • Anti-SOX9 antibody [3C10] (ab76997) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size: 56 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?



    This image was generated using the ascites version of the product.

     

  • Anti-SOX9 antibody [3C10] (ab76997) at 5 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa



    This image was generated using the ascites version of the product.

     

  • Immunoprecipitation of SOX9 transfected lysate using anti-SOX9 monoclonal antibody and Protein A magnetic beads. Western blot was performed with an rabbit anti-SOX9 polyclonal antibody.

    This image was generated using the ascites version of the product.

     

References

This product has been referenced in:
  • Sherman-Samis M  et al. SOX2 and SOX9 are markers of clinically aggressive disease in metastatic high-grade serous carcinoma. Gynecol Oncol 153:651-660 (2019). Read more (PubMed: 30904337) »
  • Tata A  et al. Myoepithelial Cells of Submucosal Glands Can Function as Reserve Stem Cells to Regenerate Airways after Injury. Cell Stem Cell 22:668-683.e6 (2018). Read more (PubMed: 29656943) »
See all 23 Publications for this product

Customer reviews and Q&As

1-10 of 16 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Blocking step
Normal Goat Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Mouse Tissue sections (Spinal Cord)
Specification
Spinal Cord
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 27 2014

Question
Answer

I have processed your request to receive ab118892 as a replacement for ab76997. Your new order number is ******, and the antibody will ship out as soon as it comes into stock.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Zebrafish Cell (24 hpf embryos)
Permeabilization
Yes - 0.5% Triton
Specification
24 hpf embryos
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Apr 15 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Bone Marrow MSCs)
Permeabilization
Yes - 0.1% Triton
Specification
Bone Marrow MSCs
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Apr 09 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Bone/Cartilage)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
Bone/Cartilage
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde

Mr. Chris Erickson

Verified customer

Submitted Mar 02 2018

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (cortical neurons)
Specification
cortical neurons
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 06 2016

Application
Immunocytochemistry
Sample
Human Cultured Cells (neural precourser cell derived from iPSC)
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Specification
neural precourser cell derived from iPSC
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 27 2016

Application
IHC - Wholemount
Sample
Human Tissue (Liver)
Specification
Liver

Abcam user community

Verified customer

Submitted Nov 16 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (muller Cell, Retina)
Specification
muller Cell, Retina
Permeabilization
Yes - triton X
Fixative
Paraformaldehyde

Dr. Ryan Macdonald

Verified customer

Submitted Aug 19 2014

Application
ELISA
Sample
Human Recombinant protein (PSYPPITRSQ peptide)
Specification
PSYPPITRSQ peptide
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 22°C
Type
Direct

Abcam user community

Verified customer

Submitted Mar 14 2013

1-10 of 16 Abreviews or Q&A

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