Overview

  • Product name
    Anti-SOX9 antibody [3C10]
    See all SOX9 primary antibodies
  • Description
    Mouse monoclonal [3C10] to SOX9
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: EQLSPSHYSE QQQHSPQQIA YSPFNLPHYS PSYPPITRSQ YDYTDHQNSS SYYSHAAGQG TGLYSTFTYM NPAQRPMYTP IADTSGVPSI PQTHSPQHWE QPVYTQLTRP, corresponding to amino acids 400-509 of human SOX9 (NP_000337) with a 26 kDa tag

  • Positive control
    • WB: HepG2 whole cell lysate (ab7900). IHC-P: Human tonsil tissue. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 22/03/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Applications

Our Abpromise guarantee covers the use of ab76997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
ELISA Use at an assay dependent concentration. Detection limit for ab76997 is approximately 30 ng/ml when used as a capture antibody.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.

Target

  • Function
    Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
  • Involvement in disease
    Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • campomelic dysplasia autosomal sex reversal antibody
    • CMD 1 antibody
    • CMD1 antibody
    • CMPD 1 antibody
    • CMPD1 antibody
    • SOX 9 antibody
    • Sox9 antibody
    • SOX9_HUMAN antibody
    • SRA 1 antibody
    • SRA1 antibody
    • SRXX2 antibody
    • SRXY10 antibody
    • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
    • SRY (sex determining region Y) box 9 antibody
    • SRY (sex determining region Y)-box 9 antibody
    • SRY (sex-determining region Y)-box 9 protein antibody
    • SRY related HMG box gene 9 antibody
    • Transcription factor SOX 9 antibody
    • Transcription factor SOX-9 antibody
    • transcription factor SOX9 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling SOX9 with ab76997 at 0.7 µg/ml.

    This image was generated using the ascites version of the product.

     

  • Immunocytochemistry/Immunofluorescence analysis of SOX9 expression in HepG2 cells, using 10 µg/ml of ab76997.

    This image was generated using the ascites version of the product.

     

  • Overlay histogram showing HepG2 cells stained with ab76997 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76997, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated using the ascites version of the product.

     

  • Anti-SOX9 antibody [3C10] (ab76997) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size: 56 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?



    This image was generated using the ascites version of the product.

     

  • Anti-SOX9 antibody [3C10] (ab76997) at 5 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa



    This image was generated using the ascites version of the product.

     

  • Immunoprecipitation of SOX9 transfected lysate using anti-SOX9 monoclonal antibody and Protein A magnetic beads. Western blot was performed with an rabbit anti-SOX9 polyclonal antibody.

    This image was generated using the ascites version of the product.

     

References

This product has been referenced in:
  • Sherman-Samis M  et al. SOX2 and SOX9 are markers of clinically aggressive disease in metastatic high-grade serous carcinoma. Gynecol Oncol 153:651-660 (2019). Read more (PubMed: 30904337) »
  • Ahmad ST  et al. Capicua regulates neural stem cell proliferation and lineage specification through control of Ets factors. Nat Commun 10:2000 (2019). Read more (PubMed: 31043608) »
See all 24 Publications for this product

Customer reviews and Q&As

1-6 of 6 Q&A

Question
Answer

I have processed your request to receive ab118892 as a replacement for ab76997. Your new order number is ******, and the antibody will ship out as soon as it comes into stock.

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Question
Answer

Thank you very much for your interest in ab76997

To our knowledge, ab/xxx/ has not been tested in Flow Cytometry. Therefore, I can offer a discount off a future purchase if you buy ab76997 now, test it in Flow Cyto and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free (PRIMARY ANTIBODY/PROTEIN) OR VALUE OFF OF NEXT ORDER.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab76997 in Flow Cyto. I will then send a discount code. This code must be issued before purchasing ab76997 so please wait for my reply before ordering.

2. Purchase ab76997 either by phone, fax, or online (www.abcam.com).

3. Test it in Flow Cyto.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any [PRIMARY ANTIBODY /PROTEIN /PRODUCT] ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab76997 turns out to be unsuitable for Flow Cytometry, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Question
Answer

DISCOUNT CODE: ***
Expiration date: October 8, 2012

I am very pleased to hear you would like to accept our offer and test ab76997 in mouse samples. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forab76997 with mouseand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

I would be happy to send you ab118892 as a replacement; it is however on back order for 2 weeks. Would you like to wait or would you like to select a different antibody? Also I believe I have located the original order, but could you just confirm that the antibody was ordered on ********. I look forward to your reply and helping resolve this issue for you.

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Answer

The antibody, ab76997 is recognizing a non-specific band and I was trying to see if we had any information on what that band could be. I have not been able to find any information on the non-specific band and so I am not sure what it could be. As you mentioned in your previous email, as it is recognizing this non-specific band the antibody is unsuitable for you to carry out the assay that you originally purchased it for. Therefore I can send a replacement SOX9 antibody free of charge. Looking through our catalogue, there are a few potential antibodies that would be suitable replacements, these are:ab58191, ab3697.

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