Hypoxic sample (treated with 0.5% oxygen for 16h). Predicted to be induced compared to normoxic sample based on mRNA analysis.
Lane 1 and 3 = MCF-7 (16h 0.5% O2)
Lane 2 and 4 = MCF-7 (Normoxia)
Lysis buffer: Urea/SDS buffer
PVDF membrane used
Bands detected at the right size but no clear induction observed in hypoxic sample. There are multiple non-specific bands observed.
Abcam user community
Submitted Nov 05 2013
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