Product nameAnti-SOX9 antibody
See all SOX9 primary antibodies
DescriptionRabbit polyclonal to SOX9
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human SOX9 aa 1-150 (N terminal). The exact sequence is proprietary.
Database link: P48436
- SW480 cells. Human testis.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab36748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
FunctionPlays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Involvement in diseaseDefects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
- Information by UniProt
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
This product has been referenced in:
- Shakhova O et al. Antagonistic cross-regulation between Sox9 and Sox10 controls an anti-tumorigenic program in melanoma. PLoS Genet 11:e1004877 (2015). IHC . Read more (PubMed: 25629959) »
- Harris ML et al. Sox proteins in melanocyte development and melanoma. Pigment Cell Melanoma Res 23:496-513 (2010). Read more (PubMed: 20444197) »