Anti-SOX9 antibody - ChIP Grade (ab3697)

Rabbit polyclonal SOX9 antibody. Validated in WB, IP, ELISA, IHC, ChIP, ICC/IF and tested in Mouse, Rat, Human. Cited in 41 publication(s). Independently reviewed in 8 review(s).

Overview

  • Product name
    Anti-SOX9 antibody - ChIP Grade
    See all SOX9 primary antibodies
  • Description
    Rabbit polyclonal to SOX9 - ChIP Grade
  • Host species
    Rabbit
  • Specificity
    The antibodies were evaluated for specificity with a dot blot assay using synthetically prepared SOX9 peptides. By immunoblot analysis, a ~65 kDa immunoreactive protein from mouse embryo cell lysate was detected (sample were boiled prior to subject 10% SDS-polyacrylamide gel electrophoresis). This band is abolished by the depletion of the synthetic peptide.
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, WB, IP, ELISA, IHC-P, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Synthetic peptide:

    KYQPRRRKSVKNG

    , corresponding to amino acids 173 - 185 of Human/ Mouse/ Bovine SOX 9.

  • Positive control
    • 3T3 cells FFPE mouse e14 whole foetus tissue sections

Applications

Our Abpromise guarantee covers the use of ab3697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200. PubMed: 19388005
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 56 kDa).
IP Use 3-5µg for 107 cells.
ELISA Use a concentration of 0.1 - 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP Use at an assay dependent concentration. PubMed: 21872590

Target

  • Function
    Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
  • Involvement in disease
    Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • campomelic dysplasia autosomal sex reversal antibody
    • CMD 1 antibody
    • CMD1 antibody
    • CMPD 1 antibody
    • CMPD1 antibody
    • SOX 9 antibody
    • Sox9 antibody
    • SOX9_HUMAN antibody
    • SRA 1 antibody
    • SRA1 antibody
    • SRXX2 antibody
    • SRXY10 antibody
    • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
    • SRY (sex determining region Y) box 9 antibody
    • SRY (sex determining region Y)-box 9 antibody
    • SRY (sex-determining region Y)-box 9 protein antibody
    • SRY related HMG box gene 9 antibody
    • Transcription factor SOX 9 antibody
    • Transcription factor SOX-9 antibody
    • transcription factor SOX9 antibody
    see all

Images

  • ChIP analysis of 15P-1 cells using no antibody (lane 1), a control rabbit IgG (lane 2) or ab3697 (lane 3). DNA from the Er71 promoter (-334 to +28), an Er71 region ranging from exon 6 to exon 7, or the 3'-UTR of Sox9 (2664–3023) was PCR amplified.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin hair follicles labling SOX9 with ab3697. Hematoxylin purple nuclear conterstain.

  • IHC-P image of SOX9 (ab3697) on E11.5 mouse embryo sections. The sections were fixed in paraformaldehyde and underwent heat mediated antigen retrieval using Tris/EGTA (pH9). The sections were then blocked in 1% BSA solution for 30 mins at 20°C. In these images, ab3697 can be seen staining nuclei on the lumen surface of the brain (green).

    See Abreview

  • Rabbit polyclonal antibody to Sox9 recognizes full length of Sox9 (left lane) and C-terminal deletion of Sox9 (right lane)

    (Compared to anti-Sox9 antibody from a different company - not shown).

    This Western blot image was kindly supplied as part of the review submitted by Lilia Topol.

  • Immunofluroescence analysis of murine nucleus pulposus (NP) cells, staining SOX9 with ab3697.
    Left panel = 2 day vehicle treated control
    Right panel = 2 day cyclopamine treated cells
  • IHC image of SOX9 staining in mouse e14 whole foetus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3697, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Immunofluroescence analysis of murine nucleus pulposus (NP) cells, staining SOX9 with ab3697.
    Left panel = control Shh flox mouse
    Right panel = Shh mutant mouse

References

This product has been referenced in:
  • Gallina D & Lincoln J Dynamic Expression Profiles of Sox9 in Embryonic, Post Natal, and Adult Heart Valve Cell Populations. Anat Rec (Hoboken) 302:108-116 (2019). Read more (PubMed: 30412364) »
  • Ma Z  et al. Autophagy promotes hepatic differentiation of hepatic progenitor cells by regulating the Wnt/ß-catenin signaling pathway. J Mol Histol 50:75-90 (2019). Read more (PubMed: 30604254) »
See all 42 Publications for this product

Customer reviews and Q&As

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1-8 of 8 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PC3 Prostate cancer cells)
Permeabilization
Yes - 0.1% Triton X-100
Specification
PC3 Prostate cancer cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 29 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Vascular smooth muscle cells)
Permeabilization
Yes - NP40
Specification
Vascular smooth muscle cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 31 2017

Application
CyTOF®
Sample
Mouse Cell (lung)
Specification
lung

Irit Milman

Verified customer

Submitted Feb 06 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Chondrocyte cell line)
Specification
Human Chondrocyte cell line
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1000µg/mL · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 09 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6.1
Sample
Zebrafish Tissue sections (Juvenile zebrafish head including gills)
Specification
Juvenile zebrafish head including gills
Permeabilization
Yes - 70% Methanol/3% H2O2, room temp x 20 min
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 20 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (E11.5 embryo)
Specification
E11.5 embryo
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EGTA pH=9
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Aug 07 2012

Application
Western blot
Sample
Human Tissue lysate - whole (Cartilage)
Loading amount
10 µg
Specification
Cartilage
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. John Wardale

Verified customer

Submitted Oct 29 2010

Application
Western blot
Sample
Mouse Cell lysate - whole cell (primary muscle cells)
Specification
primary muscle cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%

Abcam user community

Verified customer

Submitted Aug 15 2005

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