Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12755-50] to SOX9 - C-terminal
- Suitable for: Flow Cyt, ICC/IF, WB
- Reacts with: Human
Product nameAnti-SOX9 antibody [EPR12755-50] - C-terminal
See all SOX9 primary antibodies
DescriptionRabbit monoclonal [EPR12755-50] to SOX9 - C-terminal
Tested applicationsSuitable for: Flow Cyt, ICC/IF, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human SOX9 aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: P48436
- HepG2, SW480 and Caco2 cell lysates; Caco2 and HepG2 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab184547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).|
FunctionPlays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Involvement in diseaseDefects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
- Information by UniProt
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
All lanes : Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : Caco2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Immunofluorescence analysis of 4% paraformaldehyde-fixed Caco2 cells, labeling SOX9 with ab184547 at 1/500 dilution (red). Alexa Fluor®555-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
Immunofluorescence analysis of 4% paraformaldehyde-fixed HepG2 cells, labeling SOX9 with ab184547 at 1/500 dilution (green). Alexa Fluor®488-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
Flow cytometric analysis of HepG2 cells (2% paraformaldehyde-fixed) labeling SOX9 with ab184547 at 1/200 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
ab184547 has been referenced in 1 publication.
- Balogh E et al. Hypoxia Triggers Osteochondrogenic Differentiation of Vascular Smooth Muscle Cells in an HIF-1 (Hypoxia-Inducible Factor 1)-Dependent and Reactive Oxygen Species-Dependent Manner. Arterioscler Thromb Vasc Biol 39:1088-1099 (2019). PubMed: 31070451