Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12755] to SOX9
- Suitable for: Flow Cyt, WB, ICC/IF, IP
- Reacts with: Human
Product nameAnti-SOX9 antibody [EPR12755]
See all SOX9 primary antibodies
DescriptionRabbit monoclonal [EPR12755] to SOX9
Tested applicationsSuitable for: Flow Cyt, WB, ICC/IF, IPmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human SOX9 aa 400 to the C-terminus.
Database link: P48436
- SW480 and Caco 2 cell lysates; SW480 and Caco 2 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab182579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|WB||1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).|
|IP||1/20 - 1/80.|
FunctionPlays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Involvement in diseaseDefects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
- Information by UniProt
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
All lanes : Anti-SOX9 antibody [EPR12755] (ab182579) at 1/10000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : Caco 2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
All lanes : Anti-SOX9 antibody [EPR12755] (ab182579) at 1/80 dilution
Lane 1 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate, 10ug
Lane 2 : SW480, 350ug, +, ab182579 2ug
Lane 3 : SW480 cell lysate, 350ug + rabbit IgG (ab172730) , 2ug
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody (HRP))
Observed band size: 75 kDa why is the actual band size different from the predicted?
Immunofluorescent analysis of Caco 2 cells labeling SOX9 using ab182579 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde.
Flow Cytometry analysis of SW480 (human colorectal adenocarcinoma ) cells labeling SOX9 with purified ab182579 at 1/500 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Immunofluorescence analysis of SW480 cells labeling SOX9 using ab182579 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde.
Detection of SOX9 by Western Blot of Immunprecipitate.
Caco 2 cell lysate immunoprecipitated using ab182579 at 1/50 dilution.
A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.
ab182579 has been referenced in 1 publication.
- Huang JQ et al. SOX9 drives the epithelial-mesenchymal transition in non-small-cell lung cancer through the Wnt/ß-catenin pathway. J Transl Med 17:143 (2019). PubMed: 31060551