Overview

  • Product name

    Anti-SOX9 antibody [EPR12755]
    See all SOX9 primary antibodies
  • Description

    Rabbit monoclonal [EPR12755] to SOX9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human SOX9 aa 400 to the C-terminus.
    Database link: P48436

  • Positive control

    • SW480 and Caco 2 cell lysates; SW480 and Caco 2 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab182579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
ICC/IF 1/250.
IP 1/20 - 1/40.

Target

  • Function

    Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
  • Involvement in disease

    Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • campomelic dysplasia autosomal sex reversal antibody
    • CMD 1 antibody
    • CMD1 antibody
    • CMPD 1 antibody
    • CMPD1 antibody
    • SOX 9 antibody
    • Sox9 antibody
    • SOX9_HUMAN antibody
    • SRA 1 antibody
    • SRA1 antibody
    • SRXX2 antibody
    • SRXY10 antibody
    • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
    • SRY (sex determining region Y) box 9 antibody
    • SRY (sex determining region Y)-box 9 antibody
    • SRY (sex-determining region Y)-box 9 protein antibody
    • SRY related HMG box gene 9 antibody
    • Transcription factor SOX 9 antibody
    • Transcription factor SOX-9 antibody
    • transcription factor SOX9 antibody
    see all

Images

  • All lanes : Anti-SOX9 antibody [EPR12755] (ab182579) at 1/10000 dilution

    Lane 1 : SW480 cell lysate
    Lane 2 : Caco 2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa

  • All lanes : Anti-SOX9 antibody [EPR12755] (ab182579) at 1/500 dilution

    Lane 1 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate, 10ug
    Lane 2 : SW480, 350ug, +, ab182579 2ug
    Lane 3 : SW480 cell lysate, 350ug + rabbit IgG (ab172730) , 2ug

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody (HRP))

    Observed band size: 75 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescent analysis of Caco 2 cells labeling SOX9 using ab182579 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde.

  • Flow Cytometry analysis of SW480 (human colorectal adenocarcinoma ) cells labeling SOX9 with purified ab182579 at 1/500 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunofluorescence analysis of SW480 cells labeling SOX9 using ab182579 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde.

  • Detection of SOX9 by Western Blot of Immunprecipitate.
    Caco 2 cell lysate immunoprecipitated using ab182579 at 1/50 dilution.

     A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.

References

ab182579 has not yet been referenced specifically in any publications.

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