Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Loading amount
20 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tween 0.1%
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Invitrogen, Donkey anti-Rabbit IgG (H+L) Highly Cr
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Detection
Detection method
Fluorescence (Odissey)
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: 65 kDa Non-specific: 90 - 40 kDa
Additional data
Additional Notes
Detects a band of approximately 65 kDa (predicted molecular weight: 56 kDa).
Abcam response
We would like to thank this researcher for submitting their review. Our scientific support team has contacted the user and sent a free of charge replacement for another antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Feb 17 2020