Application
IHC - Wholemount
Sample
Human Embryo (whole lung (age PCW7))
Specification
whole lung (age PCW7)
Other product details
Incubation time
99 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 0.5%Triton/0. 5% Gelatin PBS1X
Dilution
1/400
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: JacksonIR Donkey anti-Rabbit, mcr, Cy3 #711165152
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
4%PFA immersion-fixed (1 day)
Incubated for 12 days at 37°C in primary antibody
then cleared using iDisco+ protocol
Imaged with light sheet microscopy
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Alain Chedotal
Verified customer
Submitted Nov 23 2020