Recombinant Anti-SOX9 antibody [EPR14335] (ab185230)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)](https://www.abcam.com/ps/products/185/ab185230/Images/ab185230-218938-anti-sox9-antibody-epr14335-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14335] to SOX9
- Suitable for: ICC/IF, WB, IHC-P, IP, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SOX9 antibody [EPR14335]
See all SOX9 primary antibodies -
Description
Rabbit monoclonal [EPR14335] to SOX9 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, IP, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Pig, Common marmoset
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SW480 and PC3 cell lysates; IHC-P: Human, mouse and rat colon tissue. ICC/IF: SW480 cells. Flow Cyt (intra): PC3 cells. IP: SW480 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14335 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
1/250.
|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| IP |
1/60.
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| Flow Cyt (Intra) |
1/190.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
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ICC/IF
1/250. |
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WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa). |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IP
1/60. |
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Flow Cyt (Intra)
1/190. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. -
Involvement in disease
Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 403464 Dog
- Entrez Gene: 6662 Human
- Entrez Gene: 20682 Mouse
- Entrez Gene: 396840 Pig
- Entrez Gene: 140586 Rat
- Omim: 608160 Human
- SwissProt: Q7YRJ7 Dog
- SwissProt: P48436 Human
see all -
Alternative names
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)](https://www.abcam.com/ps/products/185/ab185230/Images/ab185230-218936-anti-sox9-antibody-epr14335-western-blot.jpg)
Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)Anti-SOX9 antibody [EPR14335] (ab185230) at 1/20000 dilution + SW480 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335] (ab185230)](https://www.abcam.com/ps/products/185/ab185230/Images/ab185230-218941-anti-sox9-antibody-epr14335-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335] (ab185230)Immunofluorescent analysis of SW480 cells (4% paraformaldehyd-fixed) labeling SOX9 with ab185230 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Alexa Fluor® 488 (ab196450) and Alexa Fluor® 647 (ab196184) conjugated versions are available for this clone.
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![Immunoprecipitation - Anti-SOX9 antibody [EPR14335] (ab185230)](https://www.abcam.com/ps/products/185/ab185230/Images/ab185230-260478-anti-sox9-antibody-epr14335-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SOX9 antibody [EPR14335] (ab185230)ab185230 at 1/60 immunoprecipitating SOX9 in SW480 (human colorectal adenocarcinoma) whole cell lysate observed at 56 and 75 KDa (lanes 1 and 2).
The expression pattern observed is consistent with the literature (PMID: 10682837) with an additional off-target band at 46 kD.
Lane 1 (input): SW480 whole cell lysate 10μg
Lane 2 (+): SW480 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab185230 in SW480 whole cell lysate
For western blotting, ab185230 (1/1000) was used as the primary antibody and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
![Flow Cytometry (Intracellular) - Anti-SOX9 antibody [EPR14335] (ab185230)](https://www.abcam.com/ps/products/185/ab185230/Images/ab185230-218942-anti-sox9-antibody-epr14335-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SOX9 antibody [EPR14335] (ab185230)Intracellular flow cytometric analysis of PC3 cells (2% paraformaldehyde-fixed)labeling SOX9 with ab185230 at 1/190 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Alexa Fluor®488 (ab196450) and Alexa Fluor®647 (ab196184) conjugated versions are available for this clone.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)](https://www.abcam.com/ps/products/185/ab185230/Images/ab185230-218940-anti-sox9-antibody-epr14335-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)](https://www.abcam.com/ps/products/185/ab185230/Images/ab185230-218937-anti-sox9-antibody-epr14335-western-blot.jpg)
Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)Anti-SOX9 antibody [EPR14335] (ab185230) at 1/5000 dilution + PC3 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)](https://www.abcam.com/ps/products/185/ab185230/Images/ab185230-218939-anti-sox9-antibody-epr14335-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-SOX9 antibody [EPR14335] (ab185230)](https://www.abcam.com/ps/products/185/ab185230/Images/ab185230-9-benefits-of-recombinant-antibodies.png)
Anti-SOX9 antibody [EPR14335] (ab185230)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (81)
ab185230 has been referenced in 81 publications.
- Russell JP et al. Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells. Elife 10:N/A (2021). PubMed: 33399538
- Zhang M et al. Eif2s3y Promotes the Proliferation of Spermatogonial Stem Cells by Activating ERK Signaling. Stem Cells Int 2021:6668658 (2021). PubMed: 33603791
- Ikeda Y et al. The conditional deletion of steroidogenic factor 1 (Nr5a1) in Sox9-Cre mice compromises testis differentiation. Sci Rep 11:4486 (2021). PubMed: 33627800
- Wang G et al. Glycyrrhizin Attenuates Carcinogenesis by Inhibiting the Inflammatory Response in a Murine Model of Colorectal Cancer. Int J Mol Sci 22:N/A (2021). PubMed: 33807620
- Mirra S et al. ARMCX3 Mediates Susceptibility to Hepatic Tumorigenesis Promoted by Dietary Lipotoxicity. Cancers (Basel) 13:N/A (2021). PubMed: 33807672