Recombinant
RabMAb

Anti-SOX9 antibody [EPR14335] (ab185230)

Overview

  • Product name
    Anti-SOX9 antibody [EPR14335]
    See all SOX9 primary antibodies
  • Description
    Rabbit monoclonal [EPR14335] to SOX9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human SOX9 aa 150-300. The exact sequence is proprietary.
    Database link: P48436

  • Positive control
    • SW480 and PC3 cell lysates; Human colon, Rat colon and Mouse colon tissues; SW480 cells; PC3 cells.
  • General notes

     

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/2000.
Flow Cyt 1/190.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/60.

Target

  • Function
    Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
  • Involvement in disease
    Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • campomelic dysplasia autosomal sex reversal antibody
    • CMD 1 antibody
    • CMD1 antibody
    • CMPD 1 antibody
    • CMPD1 antibody
    • SOX 9 antibody
    • Sox9 antibody
    • SOX9_HUMAN antibody
    • SRA 1 antibody
    • SRA1 antibody
    • SRXX2 antibody
    • SRXY10 antibody
    • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
    • SRY (sex determining region Y) box 9 antibody
    • SRY (sex determining region Y)-box 9 antibody
    • SRY (sex-determining region Y)-box 9 protein antibody
    • SRY related HMG box gene 9 antibody
    • Transcription factor SOX 9 antibody
    • Transcription factor SOX-9 antibody
    • transcription factor SOX9 antibody
    see all

Images

  • Anti-SOX9 antibody [EPR14335] (ab185230) at 1/20000 dilution + SW480 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa

  • Anti-SOX9 antibody [EPR14335] (ab185230) at 1/5000 dilution + PC3 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Immunofluorescent analysis of SW480 cells (4% paraformaldehyd-fixed) labeling SOX9 with ab185230 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Flow Cytometric analysis of PC3 cells (2% paraformaldehyde-fixed) labeling SOX9 with ab185230 at 1/190 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • ab185230 at 1/60 immunoprecipitating SOX9 in SW480 (human colorectal adenocarcinoma) whole cell lysate observed at 56 and 75 KDa (lanes 1 and 2).

    The expression pattern observed is consistent with the literature (PMID: 10682837) with an additional off-target band at 46 kD.

    Lane 1 (input): SW480 whole cell lysate 10μg

    Lane 2 (+): SW480 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab185230 in SW480 whole cell lysate

    For western blotting, ab185230 (1/1000) was used as the primary antibody and ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10 000).

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

     

References

This product has been referenced in:
  • Tong YF  et al. Maturity of associating liver partition and portal vein ligation for staged hepatectomy-derived liver regeneration in a rat model. World J Gastroenterol 24:1107-1119 (2018). Read more (PubMed: 29563755) »
  • Flores A  et al. Lactate dehydrogenase activity drives hair follicle stem cell activation. Nat Cell Biol 19:1017-1026 (2017). Read more (PubMed: 28812580) »

See all 10 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Mr. Elena Ezhkova

Verified customer

Submitted Jan 22 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse testes - Sertoli cells)
Permeabilization
Yes - 0.1% Triton X-100 in PBS - 10 min
Specification
Mouse testes - Sertoli cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 16 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Florida manatee Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA + Tween20, pH 9.0
Permeabilization
No
Specification
Testis
Blocking step
Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
10% neutral buffered formalin
Username

Jonathan Cowart

Verified customer

Submitted May 23 2017

Application
ChIP
Sample
Mouse Tissue lysate - nuclear (Pancreas)
Negative control
A locus with no SOX9 enrichment/binding (intron 2 of Rpl30, primer provided in Cell Signaling SimpleChIP #9005)
Specification
Pancreas
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 20 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1.5% formaldehyde
Positive control
Three loci in gene promoter regions demonstrated to have SOX9 binding by ChIP-Seq from several previously published data sets. For loci that are within the promoter region of multiple genes both genes are listed separated by a "/".
Username

Abcam user community

Verified customer

Submitted Feb 28 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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