RecombinantRabMAb

Recombinant Anti-SOX9 antibody [EPR14335] (ab185230)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)
  • Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335] (ab185230)
  • Immunoprecipitation - Anti-SOX9 antibody [EPR14335] (ab185230)
  • Flow Cytometry (Intracellular) - Anti-SOX9 antibody [EPR14335] (ab185230)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)
  • Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)
  • Anti-SOX9 antibody [EPR14335] (ab185230)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14335] to SOX9
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IP, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-SOX9 antibody [EPR14335]
    See all SOX9 primary antibodies

  • Description

    Rabbit monoclonal [EPR14335] to SOX9

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IP, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SW480 and PC3 cell lysates; IHC-P: Human, mouse and rat colon tissue. ICC/IF: SW480 cells. Flow Cyt: PC3 cells. IP: SW480 cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
1/250.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
1/190.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP
1/60.
Flow Cyt (Intra)
1/190.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Notes
ICC/IF
1/250.
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
1/190.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP
1/60.
Flow Cyt (Intra)
1/190.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.

  • Involvement in disease

    Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.

  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • campomelic dysplasia autosomal sex reversal antibody
      • CMD 1 antibody
      • CMD1 antibody
      • CMPD 1 antibody
      • CMPD1 antibody
      • SOX 9 antibody
      • Sox9 antibody
      • SOX9_HUMAN antibody
      • SRA 1 antibody
      • SRA1 antibody
      • SRXX2 antibody
      • SRXY10 antibody
      • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
      • SRY (sex determining region Y) box 9 antibody
      • SRY (sex determining region Y)-box 9 antibody
      • SRY (sex-determining region Y)-box 9 protein antibody
      • SRY related HMG box gene 9 antibody
      • Transcription factor SOX 9 antibody
      • Transcription factor SOX-9 antibody
      • transcription factor SOX9 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)
      Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)
      Anti-SOX9 antibody [EPR14335] (ab185230) at 1/20000 dilution + SW480 cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 56 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335] (ab185230)
      Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335] (ab185230)

      Immunofluorescent analysis of SW480 cells (4% paraformaldehyd-fixed) labeling SOX9 with ab185230 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

      Alexa Fluor® 488 (ab196450) and Alexa Fluor® 647 (ab196184) conjugated versions are available for this clone.

    • Immunoprecipitation - Anti-SOX9 antibody [EPR14335] (ab185230)
      Immunoprecipitation - Anti-SOX9 antibody [EPR14335] (ab185230)

      ab185230 at 1/60 immunoprecipitating SOX9 in SW480 (human colorectal adenocarcinoma) whole cell lysate observed at 56 and 75 KDa (lanes 1 and 2).

      The expression pattern observed is consistent with the literature (PMID: 10682837) with an additional off-target band at 46 kD.

      Lane 1 (input): SW480 whole cell lysate 10μg

      Lane 2 (+): SW480 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab185230 in SW480 whole cell lysate

      For western blotting, ab185230 (1/1000) was used as the primary antibody and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

       

    • Flow Cytometry (Intracellular) - Anti-SOX9 antibody [EPR14335] (ab185230)
      Flow Cytometry (Intracellular) - Anti-SOX9 antibody [EPR14335] (ab185230)

      Flow Cytometric analysis of PC3 cells (2% paraformaldehyde-fixed) labeling SOX9 with ab185230 at 1/190 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

      Alexa Fluor® 488 (ab196450) and Alexa Fluor® 647 (ab196184) conjugated versions are available for this clone.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)

      Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)
      Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)
      Anti-SOX9 antibody [EPR14335] (ab185230) at 1/5000 dilution + PC3 cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 56 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)

      Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-SOX9 antibody [EPR14335] (ab185230)
      Anti-SOX9 antibody [EPR14335] (ab185230)

    References (55)

    Publishing research using ab185230? Please let us know so that we can cite the reference in this datasheet.

    ab185230 has been referenced in 55 publications.

    • Ishiguro KI  et al. MEIOSIN Directs the Switch from Mitosis to Meiosis in Mammalian Germ Cells. Dev Cell N/A:N/A (2020). ICC/IF ; Mouse . PubMed: 32032549
    • Vukicevic S  et al. Autologous blood coagulum is a physiological carrier for BMP6 to induce new bone formation and promote posterolateral lumbar spine fusion in rabbits. J Tissue Eng Regen Med 14:147-159 (2020). PubMed: 31671243
    • Sorkin M  et al. Regulation of heterotopic ossification by monocytes in a mouse model of aberrant wound healing. Nat Commun 11:722 (2020). PubMed: 32024825
    • Gu L  et al. Preparation and characterization of methacrylated gelatin/bacterial cellulose composite hydrogels for cartilage tissue engineering. Regen Biomater 7:195-202 (2020). PubMed: 32296538
    • Bao J  et al. Rapamycin protects chondrocytes against IL-18-induced apoptosis and ameliorates rat osteoarthritis. Aging (Albany NY) 12:5152-5167 (2020). PubMed: 32182210
    View all Publications for this product

    Customer reviews and Q&As

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    Western blot abreview for Anti-SOX9 antibody [EPR14335]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Cerebellum)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    30 µg
    Specification
    Cerebellum
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

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    Submitted Feb 02 2021

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