RecombinantRabMAb

Recombinant Anti-SOX9 antibody [EPR14335] (ab185230)

Reviews (4) Submit a question References (43)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14335] to SOX9
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SOX9 antibody [EPR14335]
    See all SOX9 primary antibodies

  • Description

    Rabbit monoclonal [EPR14335] to SOX9

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human SOX9 aa 150-300. The exact sequence is proprietary.
    Database link: P48436

  • Positive control

    • WB: SW480 and PC3 cell lysates; IHC-P: Human, mouse and rat colon tissue. ICC/IF: SW480 cells. Flow Cyt: PC3 cells. IP: SW480 cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab185230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/190.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/60.

Target

  • Function

    Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.

  • Involvement in disease

    Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.

  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • campomelic dysplasia autosomal sex reversal antibody
    • CMD 1 antibody
    • CMD1 antibody
    • CMPD 1 antibody
    • CMPD1 antibody
    • SOX 9 antibody
    • Sox9 antibody
    • SOX9_HUMAN antibody
    • SRA 1 antibody
    • SRA1 antibody
    • SRXX2 antibody
    • SRXY10 antibody
    • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
    • SRY (sex determining region Y) box 9 antibody
    • SRY (sex determining region Y)-box 9 antibody
    • SRY (sex-determining region Y)-box 9 protein antibody
    • SRY related HMG box gene 9 antibody
    • Transcription factor SOX 9 antibody
    • Transcription factor SOX-9 antibody
    • transcription factor SOX9 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)
    Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)
    Anti-SOX9 antibody [EPR14335] (ab185230) at 1/20000 dilution + SW480 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335] (ab185230)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335] (ab185230)

    Immunofluorescent analysis of SW480 cells (4% paraformaldehyd-fixed) labeling SOX9 with ab185230 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

    Alexa Fluor® 488 (ab196450) and Alexa Fluor® 647 (ab196184) conjugated versions are available for this clone.

  • Immunoprecipitation - Anti-SOX9 antibody [EPR14335] (ab185230)
    Immunoprecipitation - Anti-SOX9 antibody [EPR14335] (ab185230)

    ab185230 at 1/60 immunoprecipitating SOX9 in SW480 (human colorectal adenocarcinoma) whole cell lysate observed at 56 and 75 KDa (lanes 1 and 2).

    The expression pattern observed is consistent with the literature (PMID: 10682837) with an additional off-target band at 46 kD.

    Lane 1 (input): SW480 whole cell lysate 10μg

    Lane 2 (+): SW480 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab185230 in SW480 whole cell lysate

    For western blotting, ab185230 (1/1000) was used as the primary antibody and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

     

  • Flow Cytometry - Anti-SOX9 antibody [EPR14335] (ab185230)
    Flow Cytometry - Anti-SOX9 antibody [EPR14335] (ab185230)

    Flow Cytometric analysis of PC3 cells (2% paraformaldehyde-fixed) labeling SOX9 with ab185230 at 1/190 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

    Alexa Fluor® 488 (ab196450) and Alexa Fluor® 647 (ab196184) conjugated versions are available for this clone.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)
    Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)
    Anti-SOX9 antibody [EPR14335] (ab185230) at 1/5000 dilution + PC3 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-SOX9 antibody [EPR14335] (ab185230)
    Anti-SOX9 antibody [EPR14335] (ab185230)

References (43)

Publishing research using ab185230? Please let us know so that we can cite the reference in this datasheet.

ab185230 has been referenced in 43 publications.

  • Ishiguro KI  et al. MEIOSIN Directs the Switch from Mitosis to Meiosis in Mammalian Germ Cells. Dev Cell N/A:N/A (2020). ICC/IF ; Mouse . PubMed: 32032549
  • Vukicevic S  et al. Autologous blood coagulum is a physiological carrier for BMP6 to induce new bone formation and promote posterolateral lumbar spine fusion in rabbits. J Tissue Eng Regen Med 14:147-159 (2020). PubMed: 31671243
  • Sorkin M  et al. Regulation of heterotopic ossification by monocytes in a mouse model of aberrant wound healing. Nat Commun 11:722 (2020). PubMed: 32024825
  • Gu L  et al. Preparation and characterization of methacrylated gelatin/bacterial cellulose composite hydrogels for cartilage tissue engineering. Regen Biomater 7:195-202 (2020). PubMed: 32296538
  • Bao J  et al. Rapamycin protects chondrocytes against IL-18-induced apoptosis and ameliorates rat osteoarthritis. Aging (Albany NY) 12:5152-5167 (2020). PubMed: 32182210
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-SOX9 antibody [EPR14335]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Mr. Elena Ezhkova

Verified customer

Submitted Jan 22 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-SOX9 antibody [EPR14335]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse testes - Sertoli cells)
Permeabilization
Yes - 0.1% Triton X-100 in PBS - 10 min
Specification
Mouse testes - Sertoli cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jun 16 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOX9 antibody [EPR14335]

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Florida manatee Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA + Tween20, pH 9.0
Permeabilization
No
Specification
Testis
Blocking step
Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
10% neutral buffered formalin
Read More

Jonathan Cowart

Verified customer

Submitted May 23 2017

ChIP abreview for Anti-SOX9 antibody [EPR14335]

 Excellent
Abreviews
abreview image
Application
ChIP
Sample
Mouse Tissue lysate - nuclear (Pancreas)
Negative control
A locus with no SOX9 enrichment/binding (intron 2 of Rpl30, primer provided in Cell Signaling SimpleChIP #9005)
Specification
Pancreas
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 20 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1.5% formaldehyde
Positive control
Three loci in gene promoter regions demonstrated to have SOX9 binding by ChIP-Seq from several previously published data sets. For loci that are within the promoter region of multiple genes both genes are listed separated by a "/".
Read More

Abcam user community

Verified customer

Submitted Feb 28 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com