Name: Anti-SOX9 antibody [EPR14335]
Brand: RabMAb
SKU: ab185230
Rating: 5 (4 reviews)

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Primary

Secondary

This product Rabbit Anti-SOX9 antibody [EPR14335] (ab185230)
ICC/IF, WB, IHC-P, Flow Cyt, IP

Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC-P, WB, ELISA, IP

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Product name

Anti-SOX9 antibody [EPR14335]
See all SOX9 primary antibodies
Description

Rabbit monoclonal [EPR14335] to SOX9
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment within Human SOX9 aa 150-300. The exact sequence is proprietary.
Database link: P48436

Run BLAST with Run BLAST with
Positive control
- WB: SW480 and PC3 cell lysates; IHC-P: Human, rat colon and mouse colon tissues; ICC/IF: SW480 cells. Flow Cyt: PC3 cells. IP: SW480 cell lysate.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14335
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit (ab102918)
- APC Conjugation Kit (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- PC3 whole cell lysate (ab3954)
Recombinant Protein
- Recombinant Human SOX9 protein (ab131911)
Related Products
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
- Recombinant Human SOX9 protein (ab131911)

Our Abpromise guarantee covers the use of ab185230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		1/250.
WB		1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
IHC-P		1/2000.
Flow Cyt		1/190. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP		1/60.

Function

Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Involvement in disease

Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
Sequence similarities

Contains 1 HMG box DNA-binding domain.
Cellular localization

Nucleus.
Target information above from: UniProt accession P48436 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6662 Human
- Entrez Gene: 20682 Mouse
- Entrez Gene: 140586 Rat
- Omim: 608160 Human
- SwissProt: P48436 Human
- SwissProt: Q04887 Mouse
- Unigene: 647409 Human
- Unigene: 286407 Mouse
- Unigene: 95086 Rat
see all
Alternative names
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
- CMPD 1 antibody
- CMPD1 antibody
- SOX 9 antibody
- Sox9 antibody
- SOX9_HUMAN antibody
- SRA 1 antibody
- SRA1 antibody
- SRXX2 antibody
- SRXY10 antibody
- SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
- SRY (sex determining region Y) box 9 antibody
- SRY (sex determining region Y)-box 9 antibody
- SRY (sex-determining region Y)-box 9 protein antibody
- SRY related HMG box gene 9 antibody
- Transcription factor SOX 9 antibody
- Transcription factor SOX-9 antibody
- transcription factor SOX9 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)

Anti-SOX9 antibody [EPR14335] (ab185230) at 1/20000 dilution + SW480 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335] (ab185230)

Immunofluorescent analysis of SW480 cells (4% paraformaldehyd-fixed) labeling SOX9 with ab185230 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Immunoprecipitation - Anti-SOX9 antibody [EPR14335] (ab185230)

ab185230 at 1/60 immunoprecipitating SOX9 in SW480 (human colorectal adenocarcinoma) whole cell lysate observed at 56 and 75 KDa (lanes 1 and 2).

The expression pattern observed is consistent with the literature (PMID: 10682837) with an additional off-target band at 46 kD.

Lane 1 (input): SW480 whole cell lysate 10μg

Lane 2 (+): SW480 whole cell lysate.

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab185230 in SW480 whole cell lysate

For western blotting, ab185230 (1/1000) was used as the primary antibody and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Flow Cytometry - Anti-SOX9 antibody [EPR14335] (ab185230)

Flow Cytometric analysis of PC3 cells (2% paraformaldehyde-fixed) labeling SOX9 with ab185230 at 1/190 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)

Anti-SOX9 antibody [EPR14335] (ab185230) at 1/5000 dilution + PC3 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

This product has been referenced in:

Fang S et al. Osteogenic effect of bone marrow mesenchymal stem cell-derived exosomes on steroid-induced osteonecrosis of the femoral head. Drug Des Devel Ther 13:45-55 (2019). Read more (PubMed: 30587927) »
Xu S & Wu X miR-134 inhibits chondrogenic differentiation of bone marrow mesenchymal stem cells by targetting SMAD6. Biosci Rep 39:N/A (2019). Read more (PubMed: 30135141) »

See all 19 Publications for this product

Publishing research using ab185230? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Skin)

Permeabilization

Specification

Skin

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C

Fixative

Paraformaldehyde

Mr. Elena Ezhkova

Verified customer

Submitted Jan 22 2018

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Mouse testes - Sertoli cells)

Permeabilization

Yes - 0.1% Triton X-100 in PBS - 10 min

Specification

Mouse testes - Sertoli cells

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Jun 16 2017

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Florida manatee Tissue sections (Testis)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA + Tween20, pH 9.0

Permeabilization

Specification

Testis

Blocking step

Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Fixative

10% neutral buffered formalin

Jonathan Cowart

Verified customer

Submitted May 23 2017

Application

ChIP

Sample

Mouse Tissue lysate - nuclear (Pancreas)

Negative control

A locus with no SOX9 enrichment/binding (intron 2 of Rpl30, primer provided in Cell Signaling SimpleChIP #9005)

Specification

Pancreas

Detection step

Real-time PCR

Type

Cross-linking (X-ChIP)
Duration of cross-linking step: 20 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1.5% formaldehyde

Positive control

Three loci in gene promoter regions demonstrated to have SOX9 binding by ChIP-Seq from several previously published data sets. For loci that are within the promoter region of multiple genes both genes are listed separated by a "/".

Abcam user community

Verified customer

Submitted Feb 28 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Recombinant Anti-SOX9 antibody [EPR14335] (ab185230)

Overview

Properties

Associated products

Applications

Target

Images

Protocols

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

Immunohistochemistry (Frozen sections) abreview for Anti-SOX9 antibody [EPR14335]

Immunocytochemistry/ Immunofluorescence abreview for Anti-SOX9 antibody [EPR14335]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOX9 antibody [EPR14335]

ChIP abreview for Anti-SOX9 antibody [EPR14335]