Name: Anti-SOX9 antibody [EPR14335]
Brand: RabMAb
SKU: ab185230
Rating: 5 (6 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14335] to SOX9
Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-SOX9 antibody [EPR14335]
See all SOX9 primary antibodies
Description

Rabbit monoclonal [EPR14335] to SOX9
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment within Human SOX9 aa 150-300. The exact sequence is proprietary.
Database link: P48436
Positive control
- WB: SW480 and PC3 cell lysates; IHC-P: Human, mouse and rat colon tissue. ICC/IF: SW480 cells. Flow Cyt: PC3 cells. IP: SW480 cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14335
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human SOX9 protein (ab131911)
Related Products
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
- Recombinant Human SOX9 protein (ab131911)

Our Abpromise guarantee covers the use of ab185230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		1/250.
WB		1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
IHC-P		1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt		1/190. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP		1/60.

Function

Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Involvement in disease

Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
Sequence similarities

Contains 1 HMG box DNA-binding domain.
Cellular localization

Nucleus.
Target information above from: UniProt accession P48436 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6662 Human
- Entrez Gene: 20682 Mouse
- Entrez Gene: 140586 Rat
- Omim: 608160 Human
- SwissProt: P48436 Human
- SwissProt: Q04887 Mouse
- Unigene: 647409 Human
- Unigene: 286407 Mouse
- Unigene: 95086 Rat
see all
Alternative names
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
- CMPD 1 antibody
- CMPD1 antibody
- SOX 9 antibody
- Sox9 antibody
- SOX9_HUMAN antibody
- SRA 1 antibody
- SRA1 antibody
- SRXX2 antibody
- SRXY10 antibody
- SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
- SRY (sex determining region Y) box 9 antibody
- SRY (sex determining region Y)-box 9 antibody
- SRY (sex-determining region Y)-box 9 protein antibody
- SRY related HMG box gene 9 antibody
- Transcription factor SOX 9 antibody
- Transcription factor SOX-9 antibody
- transcription factor SOX9 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)

Anti-SOX9 antibody [EPR14335] (ab185230) at 1/20000 dilution + SW480 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335] (ab185230)

Immunofluorescent analysis of SW480 cells (4% paraformaldehyd-fixed) labeling SOX9 with ab185230 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor^® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Alexa Fluor^® 488 (ab196450) and Alexa Fluor^® 647 (ab196184) conjugated versions are available for this clone.
Immunoprecipitation - Anti-SOX9 antibody [EPR14335] (ab185230)

ab185230 at 1/60 immunoprecipitating SOX9 in SW480 (human colorectal adenocarcinoma) whole cell lysate observed at 56 and 75 KDa (lanes 1 and 2).

The expression pattern observed is consistent with the literature (PMID: 10682837) with an additional off-target band at 46 kD.

Lane 1 (input): SW480 whole cell lysate 10μg

Lane 2 (+): SW480 whole cell lysate.

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab185230 in SW480 whole cell lysate

For western blotting, ab185230 (1/1000) was used as the primary antibody and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Flow Cytometry - Anti-SOX9 antibody [EPR14335] (ab185230)

Flow Cytometric analysis of PC3 cells (2% paraformaldehyde-fixed) labeling SOX9 with ab185230 at 1/190 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Alexa Fluor^® 488 (ab196450) and Alexa Fluor^® 647 (ab196184) conjugated versions are available for this clone.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-SOX9 antibody [EPR14335] (ab185230)

Anti-SOX9 antibody [EPR14335] (ab185230) at 1/5000 dilution + PC3 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] (ab185230)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-SOX9 antibody [EPR14335] (ab185230)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Publishing research using ab185230? Please let us know so that we can cite the reference in this datasheet.

ab185230 has been referenced in 43 publications.

Ishiguro KI et al. MEIOSIN Directs the Switch from Mitosis to Meiosis in Mammalian Germ Cells. Dev Cell N/A:N/A (2020). ICC/IF ; Mouse . PubMed: 32032549
Vukicevic S et al. Autologous blood coagulum is a physiological carrier for BMP6 to induce new bone formation and promote posterolateral lumbar spine fusion in rabbits. J Tissue Eng Regen Med 14:147-159 (2020). PubMed: 31671243
Sorkin M et al. Regulation of heterotopic ossification by monocytes in a mouse model of aberrant wound healing. Nat Commun 11:722 (2020). PubMed: 32024825
Gu L et al. Preparation and characterization of methacrylated gelatin/bacterial cellulose composite hydrogels for cartilage tissue engineering. Regen Biomater 7:195-202 (2020). PubMed: 32296538
Bao J et al. Rapamycin protects chondrocytes against IL-18-induced apoptosis and ameliorates rat osteoarthritis. Aging (Albany NY) 12:5152-5167 (2020). PubMed: 32182210

View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Mouse Tissue sections (Brain (hippocampus))

Antigen retrieval step

None

Permeabilization

Yes - PBS 1x, Triton X-100 1%

Specification

Brain (hippocampus)

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 14 2020

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Rat Tissue sections (Cerebellum)

Antigen retrieval step

None

Permeabilization

Yes - PBS 1x, Triton X-100 1%

Specification

Cerebellum

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 14 2020

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Skin)

Permeabilization

Specification

Skin

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C

Fixative

Paraformaldehyde

Mr. Elena Ezhkova

Verified customer

Submitted Jan 22 2018

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Mouse testes - Sertoli cells)

Permeabilization

Yes - 0.1% Triton X-100 in PBS - 10 min

Specification

Mouse testes - Sertoli cells

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Jun 16 2017

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Florida manatee Tissue sections (Testis)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA + Tween20, pH 9.0

Permeabilization

Specification

Testis

Blocking step

Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Fixative

10% neutral buffered formalin

Jonathan Cowart

Verified customer

Submitted May 23 2017

Application

ChIP

Sample

Mouse Tissue lysate - nuclear (Pancreas)

Negative control

A locus with no SOX9 enrichment/binding (intron 2 of Rpl30, primer provided in Cell Signaling SimpleChIP #9005)

Specification

Pancreas

Detection step

Real-time PCR

Type

Cross-linking (X-ChIP)
Duration of cross-linking step: 20 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1.5% formaldehyde

Positive control

Three loci in gene promoter regions demonstrated to have SOX9 binding by ChIP-Seq from several previously published data sets. For loci that are within the promoter region of multiple genes both genes are listed separated by a "/".

Abcam user community

Verified customer

Submitted Feb 28 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

Recombinant Protein

Related Products

Applications

Target

Function

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Certificate of Compliance

References (43)

Customer reviews and Q&As

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Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX9 antibody [EPR14335]

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX9 antibody [EPR14335]

Immunohistochemistry (Frozen sections) abreview for Anti-SOX9 antibody [EPR14335]

Immunocytochemistry/ Immunofluorescence abreview for Anti-SOX9 antibody [EPR14335]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOX9 antibody [EPR14335]

ChIP abreview for Anti-SOX9 antibody [EPR14335]