Product nameAnti-SOX9 (phospho S181) antibody
See all SOX9 primary antibodies
DescriptionRabbit polyclonal to SOX9 (phospho S181)
SpecificityDetects endogenous levels of SOX9 only when phosphorylated at serine 181.
Tested applicationsSuitable for: ELISA, IHC-P, WB, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Mouse, Chicken, Human
Synthetic peptide corresponding to Human SOX9. Synthetic phosphopeptide derived from human SOX9 around the phosphorylation site of serine 181 (R-K-SP-V-K).
Database link: P48436
- IHC-P: Human brain. WB: HepG2 treated with UV, 293 cells treated with PBS.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab59252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 23382206|
|ICC/IF||1/100 - 1/500.|
FunctionPlays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Involvement in diseaseDefects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
- Information by UniProt
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
All lanes : Anti-SOX9 (phospho S181) antibody (ab59252) at 1/5000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate treated with UV
Lysates/proteins at 50 µg per lane.
All lanes : Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution
Predicted band size: 56 kDa
Loading control = GAPDH
Primary incubation: 4°C overnight
ab59252 at 1/50 dilution staining SOX9 in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
All lanes : Anti-SOX9 (phospho S181) antibody (ab59252) at 1/500 dilution
Lane 1 : 293 cell extracts treated
with PBS (60mins)
Lane 2 : 293 cell extracts treated
with PBS (60mins) with immunizing phospho-peptide
Predicted band size: 56 kDa
Observed band size: 56 kDa
This product has been referenced in:
- Gallina D & Lincoln J Dynamic Expression Profiles of Sox9 in Embryonic, Post Natal, and Adult Heart Valve Cell Populations. Anat Rec (Hoboken) 302:108-116 (2019). Read more (PubMed: 30412364) »
- Lam CJ et al. Highly Proliferative a-Cell-Related Islet Endocrine Cells in Human Pancreata. Diabetes 67:674-686 (2018). Read more (PubMed: 29326366) »