Product nameAnti-SP-C antibody [EPR19839]
See all SP-C primary antibodies
DescriptionRabbit monoclonal [EPR19839] to SP-C
Tested applicationsSuitable for: WB, IHC-Fr, IP, IHC-Pmore details
Species reactivityReacts with: Mouse
This product was produced with the following immunogens:
Synthetic peptide within Mouse SP-C aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P21841
Synthetic peptide within Mouse SP-C aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P21841
- WB: Mouse lung lysate. IHC-P: Mouse lung tissue. IHC-Fr: Mouse lung tissue. IP: Mouse lung lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab211326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).|
Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).
|IHC-P||1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionPulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
Involvement in diseaseDefects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'.
Sequence similaritiesContains 1 BRICHOS domain.
Cellular localizationSecreted > extracellular space > surface film.
- Information by UniProt
- BRICD6 antibody
- BRICHOS domain containing 6 antibody
- PSP C antibody
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling SP-C with ab211326 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID: 23967208].
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling SP-C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID: 15186480].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
All lanes : Anti-SP-C antibody [EPR19839] (ab211326) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Mouse spleen lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 5 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
The banding pattern in Lane 1 represents pro- and mature forms of SP-C.
Negative control: mouse spleen. [PMID: 15186480].
SP-C was immunoprecipitated from 0.35 mg of mouse lung lysate with ab211326 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab211326 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: Mouse lung lysate, 10ug (Input).
Lane 2: ab211326 IP in mouse lung lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211326 in mouse lung lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
The banding pattern represents pro- and mature forms of SFTPC.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling SP-C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative control: no staining on mouse spleen. [PMID: 15186480]. Counter stained with Hematoxylin.