Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SP-C antibody [EPR19839]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung Tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6 Citrate
Permeabilization
Yes - 0.1% Tween 20
Specification
Lung Tissue
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
10% NBF

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% bSA in PBS
Dilution
1/1000

Secondary antibody

Dilution
1/250
Name
Non-Abcam antibody was used: Donkey anti Rabbit alexa 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

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Submitted Feb 26 2018

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