Recombinant
RabMAb

Recombinant Anti-SP-C antibody [EPR19839] - Low endotoxin, Azide free (ab222929)

Overview

  • Product name

    Anti-SP-C antibody [EPR19839] - Low endotoxin, Azide free
    See all SP-C primary antibodies
  • Description

    Rabbit monoclonal [EPR19839] to SP-C - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-Fr, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P21841

    Synthetic peptide aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P21841

  • Positive control

    • WB: Mouse lung lysate. IHC-P: Mouse lung tissue. IHC-Fr: Mouse lung tissue. IP: Mouse lung lysate.
  • General notes

    ab222929 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19839
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab222929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).

WB Use at an assay dependent concentration. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
  • Involvement in disease

    Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
    Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'.
  • Sequence similarities

    Contains 1 BRICHOS domain.
  • Cellular localization

    Secreted > extracellular space > surface film.
  • Information by UniProt
  • Database links

  • Alternative names

    • BRICD6 antibody
    • BRICHOS domain containing 6 antibody
    • PSP C antibody
    • PSPC_HUMAN antibody
    • Pulmonary surfactant apoprotein 2 SP C antibody
    • Pulmonary surfactant apoprotein PSP C antibody
    • Pulmonary surfactant associated protein C antibody
    • Pulmonary surfactant associated proteolipid SPL(Val) antibody
    • Pulmonary surfactant protein SP5 antibody
    • Pulmonary surfactant-associated protein C antibody
    • Pulmonary surfactant-associated proteolipid SPL(Val) antibody
    • SFTP 2 antibody
    • SFTP2 antibody
    • SFTPC antibody
    • SMDP2 antibody
    • SP C antibody
    • SP-C antibody
    • SP5 antibody
    • Surfactant associated protein C antibody
    • Surfactant associated protein pulmonary 2 antibody
    • Surfactant protein C antibody
    • Surfactant proteolipid SPL pVal antibody
    • Surfactant pulmonary associated protein C antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling SP-C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID: 15186480].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling SP-C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on mouse spleen. [PMID: 15186480]. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling SP-C with ab211326  at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID: 23967208].

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

  • SP-C was immunoprecipitated from 0.35 mg of mouse lung lysate with ab211326 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab211326 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse lung lysate, 10ug (Input).

    Lane 2: ab211326 IP in mouse lung lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211326 in mouse lung lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    The banding pattern represents pro- and mature forms of SP-C.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

References

ab222929 has not yet been referenced specifically in any publications.

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