Product nameAnti-SP1 antibody
See all SP1 primary antibodies
DescriptionRabbit polyclonal to SP1
Tested applicationsSuitable for: WB, IP, IHC-P, ChIP, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Rhesus monkey
Recombinant fragment within Human SP1 (internal sequence). The exact sequence is proprietary.
Database link: P08047
- WB: HEK-293T and THP-1 whole cell lysate. ICC/IF: HeLa cells. IHC: HeLa and C2C12 xenografts. IP: THP-1 whole cell lysate. ChIP: HEK-293T chromatin extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/20000.|
|IP||1/100 - 1/500.|
|IHC-P||1/100 - 1/1000.|
|ChIP||Use at an assay dependent concentration.|
|ICC/IF||1/100 - 1/1000.|
FunctionTranscription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression.
Tissue specificityUp-regulated in adenocarcinomas of the stomach (at protein level).
Sequence similaritiesBelongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
modificationsPhosphorylated on multiple serine and threonine residues. Phosphorylation is coupled to ubiquitination, sumoylation and proteolytic processing. Phosphorylation on Ser-59 enhances proteolytic cleavage. Phosphorylation on Ser-7 enhances ubiquitination and protein degradation. Hyperphosphorylation on Ser-101 in response to DNA damage has no effect on transcriptional activity. MAPK1/MAPK3-mediated phosphorylation on Thr-453 and Thr-739 enhances VEGF transcription but, represses FGF2-triggered PDGFR-alpha transcription. Also implicated in the repression of RECK by ERBB2. Hyperphosphorylated on Thr-278 and Thr-739 during mitosis by MAPK8 shielding SP1 from degradation by the ubiquitin-dependent pathway. Phosphorylated in the zinc-finger domain by calmodulin-activated PKCzeta. Phosphorylation on Ser-641 by PKCzeta is critical for TSA-activated LHR gene expression through release of its repressor, p107. Phosphorylation on Thr-668, Ser-670 and Thr-681 is stimulated by angiotensin II via the AT1 receptor inducing increased binding to the PDGF-D promoter. This phosphorylation is increased in injured artey wall. Ser-59 and Thr-681 can both be dephosphorylated by PP2A during cell-cycle interphase. Dephosphorylation on Ser-59 leads to increased chromatin association during interphase and increases the transcriptional activity. On insulin stimulation, sequentially glycosylated and phosphorylated on several C-terminal serine and threonine residues.
Acetylated. Acetylation/deacetylation events affect transcriptional activity. Deacetylation leads to an increase in the expression the 12(s)-lipooxygenase gene though recruitment of p300 to the promoter.
Ubiquitinated. Ubiquitination occurs on the C-terminal proteolytically-cleaved peptide and is triggered by phosphorylation.
Sumoylated by SUMO1. Sumoylation modulates proteolytic cleavage of the N-terminal repressor domain. Sumoylation levels are attenuated during tumorigenesis. Phosphorylation mediates SP1 desumoylation.
Proteolytic cleavage in the N-terminal repressor domain is prevented by sumoylation. The C-terminal cleaved product is susceptible to degradation.
O-glycosylated; contains at least 8 N-acetylglucosamine side chains. Levels are controlled by insulin and the SP1 phosphorylation states. Insulin-mediated O-glycosylation locates SP1 to the nucleus, where it is sequentially deglycosylated and phosphorylated. O-glycosylation affects transcriptional activity through disrupting the interaction with a number of transcription factors including ELF1 and NFYA. Also inhibits interaction with the HIV1 promoter. Inhibited by peroxisomome proliferator receptor gamma (PPARgamma).
Cellular localizationNucleus. Cytoplasm. Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location.
- Information by UniProt
- SP 1 antibody
- SP1 antibody
- Sp1 transcription factor antibody
All lanes : Anti-SP1 antibody (ab227383) at 1/10000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : SP1 shRNA transfected HEK-293T whole cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SP1 (green) using ab227383 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Red: phalloidin, a cytoskeleton marker, diluted at 1/200.
ChIP was performed with HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen)chromatin extract and 5 μg of either normal rabbit IgG or anti-SP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MGARP promoter.
SP1 was immunoprecipitated from THP-1 (human monocytic leukemia cell line) whole cell lysate with ab227383. Western blot was performed from the immunoprecipitate using ab227383. Anti-rabbit IgG was used as secondary antibody.
Lane 1: THP-1 whole cell lysate (Input).
Lane 2: Rabbit IgG instead of ab227383 in THP-1 whole cell lysate.
Lane 3: ab227383 IP in THP-1 whole cell lysate.
Paraffin-embedded HeLa (human epithelial cell line from cervix adenocarcinoma) xenograft stained for SP1 using ab227383 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded C2C12 (mouse myoblast cell line) xenograft stained for SP1 using ab227383 at 1/500 dilution in immunohistochemical analysis.
Anti-SP1 antibody (ab227383) at 1/2000 dilution + THP-1 (human monocytic leukemia cell line) whole cell lysate at 30 µg
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
This product has been referenced in:
- Dong H et al. Activation of LncRNA TINCR by H3K27 acetylation promotes Trastuzumab resistance and epithelial-mesenchymal transition by targeting MicroRNA-125b in breast Cancer. Mol Cancer 18:3 (2019). Read more (PubMed: 30621694) »
- Yu S et al. SP1-induced lncRNA TINCR overexpression contributes to colorectal cancer progression by sponging miR-7-5p. Aging (Albany NY) 11:1389-1403 (2019). Read more (PubMed: 30853664) »