Western blot abreview for Anti-SP1 (phospho T453) antibody - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
13 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
Chemiluminescence
Exposure
30 second(s)
Bands
Specific: 80 kDa

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Submitted Sep 21 2016

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