Overview

  • Product name

    Anti-SP100 antibody
  • Description

    Mouse polyclonal to SP100
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-480 of Human SP100 Isoform A (AAH11562.1).

  • Positive control

    • Human spleen tissue. SP100 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab167605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 100 kDa. Isoform A predicted MW 54 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    May play a role in the control of gene expression.
  • Tissue specificity

    Widely expressed. Sp100-B is expressed only in spleen, tonsil, thymus, mature B-cell line and some T-cell line, but not in brain, liver, muscle or non-lymphoid cell lines.
  • Sequence similarities

    Contains 2 HMG box DNA-binding domains.
    Contains 1 HSR domain.
    Contains 1 SAND domain.
  • Domain

    The HSR domain is important for the nuclear body targeting as well as for the dimerization.
    Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
  • Post-translational
    modifications

    Sumoylated. Sumoylation depends on a functional nuclear localization signal but is not necessary for nuclear import or nuclear body targeting.
  • Cellular localization

    Nucleus > PML body. Found in the nuclear body, also known as nuclear domain 10 (ND10), PML oncogenic domain (POD), nuclear dots (ND) and KR body. The nuclear body is a nucleoplasmic structure of punctate shape, which varies in size and number. Induction by interferon and may be cell cycle stages modulate the subnuclear localization of the isoforms.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686E07254 antibody
    • FLJ00340 antibody
    • FLJ34579 antibody
    • Lysp100b antibody
    • Nuclear antigen Sp100 antibody
    • Nuclear autoantigen Sp 100 antibody
    • Nuclear autoantigen Sp-100 antibody
    • Nuclear autoantigen Sp100 antibody
    • Nuclear dot associated Sp100 protein antibody
    • Nuclear dot-associated Sp100 protein antibody
    • SP 100 antibody
    • SP100 antibody
    • SP100 HMG nuclear autoantigen antibody
    • SP100 nuclear antigen antibody
    • SP100_HUMAN antibody
    • Speckled 100 kDa antibody
    see all

Images

  • All lanes : Anti-SP100 antibody (ab167605) at 1 µg/ml

    Lane 1 : SP100 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Predicted band size: 100 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling SP100 with ab167605 at 3 µg/ml.

References

This product has been referenced in:

  • Liu XJ  et al. Human cytomegalovirus IE1 downregulates Hes1 in neural progenitor cells as a potential E3 ubiquitin ligase. PLoS Pathog 13:e1006542 (2017). Read more (PubMed: 28750047) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Human Cell (Human Fetal Lung Fibroblast)
Specification
Human Fetal Lung Fibroblast
Permeabilization
Yes - 0.3% Trition in PBS, 10 minutes, Room temp
Fixative
Paraformaldehyde

Jacqueline Bowlin

Verified customer

Submitted Jun 09 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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