Overview

  • Product name
  • Description
    Rabbit polyclonal to SP100
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ChIPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human SP100.

    Read Abcam's proprietary immunogen policy (Peptide available as ab44037.)

  • Positive control
    • This antibody gave a positive signal in the following human lysates: HeLa Whole Cell; Ramos Whole Cell; Hodgkin's Lymphoma Whole Cell - tumor tissue; Lymphoma Whole Cell - tumor tissue; MCF7 Whole Cell and ZR-75-1 Nuclear.

Properties

Applications

Our Abpromise guarantee covers the use of ab43151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
ChIP
  • Application notes
    IHC-P: 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (see Abreview).
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 100 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May play a role in the control of gene expression.
    • Tissue specificity
      Widely expressed. Sp100-B is expressed only in spleen, tonsil, thymus, mature B-cell line and some T-cell line, but not in brain, liver, muscle or non-lymphoid cell lines.
    • Sequence similarities
      Contains 2 HMG box DNA-binding domains.
      Contains 1 HSR domain.
      Contains 1 SAND domain.
    • Domain
      The HSR domain is important for the nuclear body targeting as well as for the dimerization.
      Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
    • Post-translational
      modifications
      Sumoylated. Sumoylation depends on a functional nuclear localization signal but is not necessary for nuclear import or nuclear body targeting.
    • Cellular localization
      Nucleus > PML body. Found in the nuclear body, also known as nuclear domain 10 (ND10), PML oncogenic domain (POD), nuclear dots (ND) and KR body. The nuclear body is a nucleoplasmic structure of punctate shape, which varies in size and number. Induction by interferon and may be cell cycle stages modulate the subnuclear localization of the isoforms.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686E07254 antibody
      • FLJ00340 antibody
      • FLJ34579 antibody
      • Lysp100b antibody
      • Nuclear antigen Sp100 antibody
      • Nuclear autoantigen Sp 100 antibody
      • Nuclear autoantigen Sp-100 antibody
      • Nuclear autoantigen Sp100 antibody
      • Nuclear dot associated Sp100 protein antibody
      • Nuclear dot-associated Sp100 protein antibody
      • SP 100 antibody
      • SP100 antibody
      • SP100 HMG nuclear autoantigen antibody
      • SP100 nuclear antigen antibody
      • SP100_HUMAN antibody
      • Speckled 100 kDa antibody
      see all

    Images

    • All lanes : Anti-SP100 antibody (ab43151) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 3 : Human lymphatic tumor tissue lysate (hodgkin's lymphoma) - total protein (ab29923)
      Lane 4 : Human lymphatic tumor tissue lysate (lymphoma) - total protein (ab30185)
      Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 6 : ZR751 nuclear extract lysate (ab14916)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 100 kDa
      Observed band size: 97 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.

    • ab43151 staining SP100 in human lymphoma tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with  peroxidase  for 5 minutes followed by a protein block for 10 minutes at 20°C . Antigen retrieval was by heat mediation in target retrieval solution. Samples were incubated with primary antibody 1/2000 for 45 minutes at 20°C. An HRP-conjugated Goat polycolonal to rabbit IgG was used as secondary antibody.

      See Abreview

    References

    This product has been referenced in:
    • McManus FP  et al. Quantitative SUMO proteomics reveals the modulation of several PML nuclear body associated proteins and an anti-senescence function of UBC9. Sci Rep 8:7754 (2018). Read more (PubMed: 29773808) »
    • Zebboudj A  et al. Sodium arsenite induces apoptosis and Epstein-Barr virus reactivation in lymphoblastoid cells. Biochimie 107 Pt B:247-56 (2014). Read more (PubMed: 25241256) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Western blot
    Loading amount
    10000 cells
    Gel Running Conditions
    Reduced Denaturing (4-12% gradient)
    Sample
    Human Cell lysate - whole cell (huh7 liver)
    Specification
    huh7 liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Dec 15 2014

    Question
    Answer

    DISCOUNT CODE: XXXXXXXX Expiration date: 15/03/2012 I am very pleased to hear you would like to accept our offer and test ab43151 in ICC/IF. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS)
    Loading amount
    35 µg
    Specification
    U2OS
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Dec 31 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (lymphoma)
    Specification
    lymphoma
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
    Permeabilization
    No
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

    Mr. Antibody Solutions

    Verified customer

    Submitted Jul 14 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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