Product nameAnti-SP100 antibody
See all SP100 primary antibodies
DescriptionRabbit polyclonal to SP100
Tested applicationsSuitable for: IHC-P, WB, ChIPmore details
Species reactivityReacts with: Human
- This antibody gave a positive signal in the following human lysates: HeLa Whole Cell; Ramos Whole Cell; Hodgkin's Lymphoma Whole Cell - tumor tissue; Lymphoma Whole Cell - tumor tissue; MCF7 Whole Cell and ZR-75-1 Nuclear.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab43151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 100 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay play a role in the control of gene expression.
Tissue specificityWidely expressed. Sp100-B is expressed only in spleen, tonsil, thymus, mature B-cell line and some T-cell line, but not in brain, liver, muscle or non-lymphoid cell lines.
Sequence similaritiesContains 2 HMG box DNA-binding domains.
Contains 1 HSR domain.
Contains 1 SAND domain.
DomainThe HSR domain is important for the nuclear body targeting as well as for the dimerization.
Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
modificationsSumoylated. Sumoylation depends on a functional nuclear localization signal but is not necessary for nuclear import or nuclear body targeting.
Cellular localizationNucleus > PML body. Found in the nuclear body, also known as nuclear domain 10 (ND10), PML oncogenic domain (POD), nuclear dots (ND) and KR body. The nuclear body is a nucleoplasmic structure of punctate shape, which varies in size and number. Induction by interferon and may be cell cycle stages modulate the subnuclear localization of the isoforms.
- Information by UniProt
- DKFZp686E07254 antibody
- FLJ00340 antibody
- FLJ34579 antibody
All lanes : Anti-SP100 antibody (ab43151) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 : Human lymphatic tumor tissue lysate (hodgkin's lymphoma) - total protein (ab29923)
Lane 4 : Human lymphatic tumor tissue lysate (lymphoma) - total protein (ab30185)
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 :
ZR751 nuclear extract lysate (ab14916)
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 100 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.
ab43151 staining SP100 in human lymphoma tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with peroxidase for 5 minutes followed by a protein block for 10 minutes at 20°C . Antigen retrieval was by heat mediation in target retrieval solution. Samples were incubated with primary antibody 1/2000 for 45 minutes at 20°C. An HRP-conjugated Goat polycolonal to rabbit IgG was used as secondary antibody.
This product has been referenced in:
- McManus FP et al. Quantitative SUMO proteomics reveals the modulation of several PML nuclear body associated proteins and an anti-senescence function of UBC9. Sci Rep 8:7754 (2018). Read more (PubMed: 29773808) »
- Zebboudj A et al. Sodium arsenite induces apoptosis and Epstein-Barr virus reactivation in lymphoblastoid cells. Biochimie 107 Pt B:247-56 (2014). Read more (PubMed: 25241256) »