For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    sp110-antibody-ab67221.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
Share by email

Anti-SP110 antibody (ab67221)

  • Datasheet
Submit a review Q&A (1)References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-SP110 antibody (ab67221)

    Key features and details

    • Rabbit polyclonal to SP110
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-SP110 antibody
      See all SP110 primary antibodies
    • Description

      Rabbit polyclonal to SP110
    • Host species

      Rabbit
    • Specificity

      This product recognizes isoforms a, b and c.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Human
    • Immunogen

      A 16 amino acid synthetic peptide derived from near the amino terminus of human SP1101. (GenBank accession no. NP_004501, isoform b).

    • Positive control

      • SW480 cell lysate
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.2
      Preservative: 0.02% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Zinc Finger
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • Nuclear Receptors
      • Co-activators/co-repressors

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    Our Abpromise guarantee covers the use of ab67221 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80-95 kDa (predicted molecular weight: 79 kDa).

    Target

    • Function

      Transcription factor. May be a nuclear hormone receptor coactivator. Enhances transcription of genes with retinoic acid response elements (RARE).
    • Tissue specificity

      Highly expressed in peripheral blood leukocytes and spleen. Detected at intermediate levels in thymus, prostate, testis, ovary, small intestine and colon, and at low levels in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • Involvement in disease

      Defects in SP110 are the cause of hepatic venoocclusive disease with immunodeficiency (VODI) [MIM:235550]. VODI is an autosomal recessive primary immunodeficiency associated with hepatic vascular occlusion and fibrosis. The immunodeficiency is characterized by severe hypogammaglobulinemia, combined T and B cell immunodeficiency, absent lymph node germinal centers, and absent tissue plasma cells.
    • Sequence similarities

      Contains 1 bromo domain.
      Contains 1 HSR domain.
      Contains 1 PHD-type zinc finger.
      Contains 1 SAND domain.
    • Post-translational
      modifications

      Phosphorylated (isoform 2).
    • Cellular localization

      Nucleus. Found in the nuclear body.
    • Target information above from: UniProt accession Q9HB58 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3431 Human
      • Entrez Gene: 109032 Mouse
      • Entrez Gene: 301570 Rat
      • Omim: 604457 Human
      • SwissProt: Q9HB58 Human
      • SwissProt: Q8BVK9 Mouse
      • SwissProt: Q3KRF1 Rat
      • Unigene: 145150 Human
      • Unigene: 335802 Mouse
      • Unigene: 461913 Mouse
      • Unigene: 6459 Rat
      see all
    • Alternative names

      • FLJ22835 antibody
      • IFI 41 antibody
      • IFI 75 antibody
      • IFI41 antibody
      • IFI75 antibody
      • Interferon induced protein 41 30kD antibody
      • Interferon induced protein 41 antibody
      • Interferon induced protein 41/75 antibody
      • Interferon induced protein 75 52kD antibody
      • Interferon induced protein 75 antibody
      • Interferon-induced protein 41/75 antibody
      • IPR 1 antibody
      • IPR1 antibody
      • Phosphoprotein 41 antibody
      • Phosphoprotein 75 antibody
      • SP 110 antibody
      • SP110 antibody
      • Sp110 nuclear body protein antibody
      • SP110_HUMAN antibody
      • Speckled 110 kDa antibody
      • Transcriptional coactivator Sp110 antibody
      • VODI antibody
      see all

    Images

    • Western blot - Anti-SP110 antibody (ab67221)
      Western blot - Anti-SP110 antibody (ab67221)
      Lane 1 : Anti-SP110 antibody (ab67221) at 1 µg/ml
      Lane 2 : Anti-SP110 antibody (ab67221) at 2 µg/ml

      All lanes : SW480 cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Anti rabbit secondary antibody

      Predicted band size: 79 kDa
      Observed band size: 80-95 kDa
      why is the actual band size different from the predicted?

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab67221? Please let us know so that we can cite the reference in this datasheet.

    ab67221 has been referenced in 1 publication.

    • Molzan M  et al. Impaired binding of 14-3-3 to C-RAF in Noonan syndrome suggests new approaches in diseases with increased Ras signaling. Mol Cell Biol 30:4698-711 (2010). PubMed: 20679480

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Can these 2 products ab67220, ab67221 detect isoform b?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 16 2008

    Answer

    Thank you for your enquiry. This product, ab67220 can detect isoform b, and c. Meanwhile, ab67221 can detect isoforms a, b,c.

    Read More

    Abcam Scientific Support

    Answered on Oct 16 2008

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.