Anti-SP110 antibody (ab67221)
Key features and details
- Rabbit polyclonal to SP110
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SP110 antibody
See all SP110 primary antibodies -
Description
Rabbit polyclonal to SP110 -
Host species
Rabbit -
Specificity
This product recognizes isoforms a, b and c. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
A 16 amino acid synthetic peptide derived from near the amino terminus of human SP1101. (GenBank accession no. NP_004501, isoform b).
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Positive control
- SW480 cell lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab67221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80-95 kDa (predicted molecular weight: 79 kDa). |
Target
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Function
Transcription factor. May be a nuclear hormone receptor coactivator. Enhances transcription of genes with retinoic acid response elements (RARE). -
Tissue specificity
Highly expressed in peripheral blood leukocytes and spleen. Detected at intermediate levels in thymus, prostate, testis, ovary, small intestine and colon, and at low levels in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
Involvement in disease
Defects in SP110 are the cause of hepatic venoocclusive disease with immunodeficiency (VODI) [MIM:235550]. VODI is an autosomal recessive primary immunodeficiency associated with hepatic vascular occlusion and fibrosis. The immunodeficiency is characterized by severe hypogammaglobulinemia, combined T and B cell immunodeficiency, absent lymph node germinal centers, and absent tissue plasma cells. -
Sequence similarities
Contains 1 bromo domain.
Contains 1 HSR domain.
Contains 1 PHD-type zinc finger.
Contains 1 SAND domain. -
Post-translational
modificationsPhosphorylated (isoform 2). -
Cellular localization
Nucleus. Found in the nuclear body. - Information by UniProt
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Database links
- Entrez Gene: 3431 Human
- Entrez Gene: 109032 Mouse
- Entrez Gene: 301570 Rat
- Omim: 604457 Human
- SwissProt: Q9HB58 Human
- SwissProt: Q8BVK9 Mouse
- SwissProt: Q3KRF1 Rat
- Unigene: 145150 Human
see all -
Alternative names
- FLJ22835 antibody
- IFI 41 antibody
- IFI 75 antibody
see all
Images
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Western blot - Anti-SP110 antibody (ab67221)Lane 1 : Anti-SP110 antibody (ab67221) at 1 µg/ml
Lane 2 : Anti-SP110 antibody (ab67221) at 2 µg/ml
All lanes : SW480 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti rabbit secondary antibody
Predicted band size: 79 kDa
Observed band size: 80-95 kDa why is the actual band size different from the predicted?
Datasheets and documents
References (1)
ab67221 has been referenced in 1 publication.
- Molzan M et al. Impaired binding of 14-3-3 to C-RAF in Noonan syndrome suggests new approaches in diseases with increased Ras signaling. Mol Cell Biol 30:4698-711 (2010). PubMed: 20679480
