Anti-SP110 antibody (ab67221)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-SP110 antibody
See all SP110 primary antibodies -
Description
Rabbit polyclonal to SP110 -
Host species
Rabbit -
Specificity
This product recognizes isoforms a, b and c. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
A 16 amino acid synthetic peptide derived from near the amino terminus of human SP1101. (GenBank accession no. NP_004501, isoform b).
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Positive control
- SW480 cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab67221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80-95 kDa (predicted molecular weight: 79 kDa). |
Target
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Function
Transcription factor. May be a nuclear hormone receptor coactivator. Enhances transcription of genes with retinoic acid response elements (RARE). -
Tissue specificity
Highly expressed in peripheral blood leukocytes and spleen. Detected at intermediate levels in thymus, prostate, testis, ovary, small intestine and colon, and at low levels in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
Involvement in disease
Defects in SP110 are the cause of hepatic venoocclusive disease with immunodeficiency (VODI) [MIM:235550]. VODI is an autosomal recessive primary immunodeficiency associated with hepatic vascular occlusion and fibrosis. The immunodeficiency is characterized by severe hypogammaglobulinemia, combined T and B cell immunodeficiency, absent lymph node germinal centers, and absent tissue plasma cells. -
Sequence similarities
Contains 1 bromo domain.
Contains 1 HSR domain.
Contains 1 PHD-type zinc finger.
Contains 1 SAND domain. -
Post-translational
modificationsPhosphorylated (isoform 2). -
Cellular localization
Nucleus. Found in the nuclear body. - Information by UniProt
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Database links
- Entrez Gene: 3431 Human
- Entrez Gene: 109032 Mouse
- Entrez Gene: 301570 Rat
- Omim: 604457 Human
- SwissProt: Q9HB58 Human
- SwissProt: Q8BVK9 Mouse
- SwissProt: Q3KRF1 Rat
- Unigene: 145150 Human
see all -
Alternative names
- FLJ22835 antibody
- IFI 41 antibody
- IFI 75 antibody
see all
Images
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Western blot - Anti-SP110 antibody (ab67221)Lane 1 : Anti-SP110 antibody (ab67221) at 1 µg/ml
Lane 2 : Anti-SP110 antibody (ab67221) at 2 µg/ml
All lanes : SW480 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti rabbit secondary antibody
Predicted band size: 79 kDa
Observed band size: 80-95 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
This product has been referenced in:
- Molzan M et al. Impaired binding of 14-3-3 to C-RAF in Noonan syndrome suggests new approaches in diseases with increased Ras signaling. Mol Cell Biol 30:4698-711 (2010). Read more (PubMed: 20679480) »
